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Western Blot (WB) (Western blot analysis of IFN-b in A-20 cell lysate with IFN-b antibody at 5 μg/mL.)

Rabbit anti-Human, Mouse IFN-beta Polyclonal Antibody | anti-IFNB1 antibody

IFN-beta Antibody

Gene Names
IFNB1; IFB; IFF; IFNB
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
IFN-beta Antibody is affinity chromatography purified via peptide column.
Synonyms
IFN-beta; Polyclonal Antibody; IFN-beta Antibody; IFB; IFF; IFNB; Interferon beta; Fibroblast interferon; interferon; beta 1; fibroblast; anti-IFNB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
IFN-beta Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
187
Applicable Applications for anti-IFNB1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IFN-b antibody can be used for detection of IFN-b by Western blot at 5 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
IFN-beta antibody was raised against a 17 amino acid synthetic peptide from near the center of human IFN-b.
Buffer
IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IFN-beta antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IFN-b in A-20 cell lysate with IFN-b antibody at 5 μg/mL.)

Western Blot (WB) (Western blot analysis of IFN-b in A-20 cell lysate with IFN-b antibody at 5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of IFN-beta in Human Liver cells with IFN-beta antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of IFN-beta in Human Liver cells with IFN-beta antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 2.5 μg/ml.)

Immunohistochemistry (IHC) (Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IFN-beta in mouse liver tissue with IFN beta antibody at 20 μg/mL.Green: IFN-beta Antibody (4243)Blue: DAPI staining)

Immunofluorescence (IF) (Immunofluorescence of IFN-beta in mouse liver tissue with IFN beta antibody at 20 μg/mL.Green: IFN-beta Antibody (4243)Blue: DAPI staining)
Related Product Information for anti-IFNB1 antibody
IFN-beta Antibody: Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by "Natural IFN-producing cells" (IPCs) as part of the host immune response and can also inhibit the development of tumors. IFN-beta binding by its receptor results in the activation of the tyrosine kinases Jak1 and Tyk2 and phosphorylation of members of the STAT family of transcription factors, leading to the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
22,294 Da
NCBI Official Full Name
interferon-beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
NCBI Protein Information
interferon beta; IFN-beta; fibroblast interferon
UniProt Protein Name
Interferon beta
Protein Family
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN

Uniprot Description

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; B cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

Research Articles on IFNB1

Similar Products

Product Notes

The IFNB1 ifnb1 (Catalog #AAA151156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFN-beta Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IFN-beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). IFN-b antibody can be used for detection of IFN-b by Western blot at 5 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the IFNB1 ifnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN-beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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