Rabbit VISA Polyclonal Antibody | anti-MAVS antibody

VISA, NT (Virus-Induced Signaling Adapter, Mitochondrial Antiviral Signaling Protein, MAVS, CARD Adapter Inducing Interferon-beta, Cardif, IPS-1)

Cat. Num. AAA615235

• Gene Names: MAVS; IPS1; VISA; IPS-1; CARDIF; MGC3260; FLJ27482; FLJ31698; FLJ35386; FLJ38051; FLJ41962; KIAA1271; DKFZp547C224; DKFZp666M015
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: VISA; Polyclonal Antibody; NT (Virus-Induced Signaling Adapter; Mitochondrial Antiviral Signaling Protein; MAVS; CARD Adapter Inducing Interferon-beta; Cardif; IPS-1); Anti -VISA; anti-MAVS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VISA. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-MAVS antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-1ug/ml. See V2124-20D for control peptide.
• Immunogen: 13 amino acid peptide from near the amino terminus of human VISA (GenBank accession no. NP_065797)
• Positive Control: A20 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2ug/ml.)

Related Product Information for anti-MAVS antibody

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response (1). VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN- signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF- B (2). VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKK, IKK and IKK kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system (3). At least three isoforms of VISA are known to exist.

Product Categories/Family for anti-MAVS antibody

Antibodies; Abs to Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 76782231
• NCBI GeneID: 57506
• UniProt Accession #: Q7Z434; Q2HWT5; Q3I0Y2; Q5T7I6; Q86VY7; Q9H1H3; Q9H4Y1; Q9H8D3; Q9ULE9
• Molecular Weight: 56,528 Da
• NCBI Official Full Name: virus-induced signaling adapter
• NCBI Official Synonym Full Names: mitochondrial antiviral signaling protein
• NCBI Official Symbol: MAVS
• NCBI Official Synonym Symbols: IPS1; VISA; IPS-1; CARDIF; MGC3260; FLJ27482; FLJ31698; FLJ35386; FLJ38051; FLJ41962; KIAA1271; DKFZp547C224; DKFZp666M015
• NCBI Protein Information: mitochondrial antiviral-signaling protein; OTTHUMP00000030141; IFN-B promoter stimulator 1; CARD adaptor inducing IFN-beta; virus-induced signaling adaptor; virus-induced-signaling adapter; CARD adapter inducing interferon beta; mitochondrial anti-viral signaling protein; putative NF-kappa-B-activating protein 031N; interferon beta promoter stimulator protein 1
• UniProt Protein Name: Mitochondrial antiviral-signaling protein
• Protein Family: Mitochondrial antiviral-signaling protein
• UniProt Gene Name: MAVS
• UniProt Synonym Gene Names: IPS1; KIAA1271; VISA
• UniProt Entry Name: MAVS_HUMAN

NCBI Description

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM]

Uniprot Description

MAVS: Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Present in T-cells, monocytes, epithelial cells and hepatocytes. Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; CARD domain binding; protein kinase binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of protein import into nucleus, translocation; positive regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-8 production; negative regulation of viral genome replication; defense response to bacterium; positive regulation of interferon-beta production; positive regulation of transcription factor import into nucleus; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of defense response to virus by host; negative regulation of interferon type I production; activation of innate immune response; defense response to virus; positive regulation of interferon-alpha production