Testing Data
(Cardif is cleaved in liver of a patient infected by the hepatitis C virus. Protein extracts of liver biopsies from a patient with chronic hepatitis C (CHC) and as a control from a patient with alcoholic hepatitis (AH) were run on a SDS-gel and revealed with anti-Cardif (human), mAb The arrows indicate endogenous human full-length or cleaved Cardif.|Picture courtesy of Prof. Darius Moradpour, CHUV, Lausanne.)
Mouse Cardif Monoclonal Antibody | anti-MAVS antibody
Cardif (CARD Adapter Inducing Interferon-beta, IPS-1, MAVS, Mitochondrial Antiviral Signaling Protein, VISA, Virus-induced Signaling Adapter, Interferon-beta Promoter Stimulator Protein 1)
>95% (SDS-PAGE)
>95% (SDS-PAGE)
Testing Data
(Cardif is cleaved in liver of a patient infected by the hepatitis C virus. Protein extracts of liver biopsies from a patient with chronic hepatitis C (CHC) and as a control from a patient with alcoholic hepatitis (AH) were run on a SDS-gel and revealed with anti-Cardif (human), mAb The arrows indicate endogenous human full-length or cleaved Cardif.|Picture courtesy of Prof. Darius Moradpour, CHUV, Lausanne.)
Testing Data
(Cardif is cleaved in liver of a patient infected by the hepatitis C virus. Protein extracts of liver biopsies from a patient with chronic hepatitis C (CHC) and as a control from a patient with alcoholic hepatitis (AH) were run on a SDS-gel and revealed with anti-Cardif (human), mAb The arrows indicate endogenous human full-length or cleaved Cardif.|Picture courtesy of Prof. Darius Moradpour, CHUV, Lausanne.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
Function: Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Ref.1 Ref.2 Ref.3 Ref.4 Ref.23 Ref.30
Subunit structure: Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6 and C1QBP. May interact with IRF3, FADD, RIPK1, CHUK and IKBKB. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1
By similarity. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Interacts with TMEM173/MITA. Interacts with IFIT3 (via N-terminus). Interacts with TBK1 only in the presence of IFIT3. Interacts with human respiratory syncytial virus (HRSV) NS1 protein; this interaction disrupts MAVS binding to DDX58/RIG-I. Ref.1 Ref.2 Ref.3 Ref.4 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.20 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.33 Ref.34
Subcellular location: Mitochondrion outer membrane. Mitochondrion. Peroxisome Ref.1 Ref.30.
Tissue specificity: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. Ref.1 Ref.2 Ref.4
Domain: Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58/RIG-I and IFIH1/MDA5.The transmembrane domain and residues 300-444 are essential for its interaction with DHX58/LGP2.
Post-translational modification: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS/IPS1 proteasomal degradation. Ref.26
Miscellaneous: Cleavage by HCV protease complex leads to inactivation.
Sequence similarities: Contains 1 CARD domain.
Sequence caution: The sequence BAA86585.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB14684.1 differs from that shown. Reason: Frameshift at position 333.
Research Articles on MAVS
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Product Notes
The MAVS mavs (Catalog #AAA648247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cardif can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot. Researchers should empirically determine the suitability of the MAVS mavs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cardif, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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