Western Blot (WB)
(Western blot analysis of cell lysates from various cell lines, using MBS618210.)
c-Abl Polyclonal Antibody | anti-c-Abl antibody
Anti - c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; bcr/abl; ABL1
Reactivity
Drosophila, Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Purified by Protein A affinity chromatography.
Synonyms
c-Abl; Polyclonal Antibody; Anti - c-Abl (ABL1; v-abl; Abelson Murine Leukemia Viral Oncogene Homolog 1; ABL; c-ABL; JTK7; p150; Proto-Oncogene Tyrosine-Protein Kanase ABL1; v-abl); c-Abl (ABL1; anti-c-Abl antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Drosophila, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects overexpressed levels of Abl proteins. The antibody detects Abl proteins immunoprecipitated from various cell lines. It does not crossreact with other related tyrosine kinase family members. Species Crossreactivity: Human, mouse and rat.
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Affinity Purified
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Sequence Length
119
Applicable Applications for anti-c-Abl antibody
ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluoroescence. Other applications not tested.
Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Recognizes bands at 135kD (c-Abl) (210kD (Bcr-Abl)).
Immunoprecipitation: 1:100
Immunofluorescence: 1:25
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Recognizes bands at 135kD (c-Abl) (210kD (Bcr-Abl)).
Immunoprecipitation: 1:100
Immunofluorescence: 1:25
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide (KLH coupled) derived from the C-terminus of human c-Abl.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of cell lysates from various cell lines, using MBS618210.)
Western Blot (WB)
(Western blot analysis of cell lysates from various cell lines, using MBS618210.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HT-29 cells labeled with MBS618210 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HT-29 cells labeled with MBS618210 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
Immunohistochemistry (IHC)
(Immunohistochemical staining of c-Abl in paraffin-embedded human breast carcinoma showing cytoplasmic localization, using c-Abl Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of c-Abl in paraffin-embedded human breast carcinoma showing cytoplasmic localization, using c-Abl Antibody.)
Related Product Information for anti-c-Abl antibody
Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in the K562 (human chronic myelogenous leukemia) cell line. The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells.
Product Categories/Family for anti-c-Abl antibody
References
• 1. Wang, J.Y. (2000) Oncogene 19, 5643-5650.
• 2. Van Etten, R.A. (1999) Trends Cell. Biol. 9, 179-182.3. Danial, N.N. and Rothman, P. (2000) Oncogene 19, 2523-2531.
• 4. Shaul, Y. (2000) Cell Death Differ. 7, 10-16.
• 5. Brasher, B.B. and Van Etten, R.A. (2000) J. Biol. Chem. 275, 35631-35637.
• 6. Pluk, H. et al. (2002) Cell 108, 247-259.
• 2. Van Etten, R.A. (1999) Trends Cell. Biol. 9, 179-182.3. Danial, N.N. and Rothman, P. (2000) Oncogene 19, 2523-2531.
• 4. Shaul, Y. (2000) Cell Death Differ. 7, 10-16.
• 5. Brasher, B.B. and Van Etten, R.A. (2000) J. Biol. Chem. 275, 35631-35637.
• 6. Pluk, H. et al. (2002) Cell 108, 247-259.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,150 Da
NCBI Official Full Name
c-abl
NCBI Official Synonym Full Names
c-abl oncogene 1, non-receptor tyrosine kinase
NCBI Official Symbol
ABL1
NCBI Official Synonym Symbols
ABL; JTK7; p150; c-ABL; v-abl; bcr/abl; ABL1
NCBI Protein Information
tyrosine-protein kinase ABL1; proto-oncogene c-Abl; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1
UniProt Protein Name
C-abl
UniProt Gene Name
ABL1
UniProt Entry Name
Q13915_HUMAN
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Product Notes
The c-Abl abl1 (Catalog #AAA618210) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl) reacts with Drosophila, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-Abl can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence. Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluoroescence. Other applications not tested. Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Recognizes bands at 135kD (c-Abl) (210kD (Bcr-Abl)). Immunoprecipitation: 1:100 Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the c-Abl abl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Abl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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