Rabbit anti-Human INCENP Polyclonal Antibody | anti-INCENP antibody

INCENP (Incenp, Inner Centromere Protein)

Cat. Num. AAA610766

• Gene Names: INCENP; FLJ31633; MGC111393
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified by peptide affinity chromatography.
• Synonyms: INCENP; Polyclonal Antibody; INCENP (Incenp; Inner Centromere Protein); Anti -INCENP (Incenp; anti-INCENP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total INCENP protein.
• Purity/Purification: Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-INCENP antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Flow Cytometry and Western Blot.; Dilution: Immunofluorescence: 1:400; Flow Cytometry: 1:50; Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Pro240 of human INCENP.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts of Jurkat and MOLT4 cells using MBS610766 INCENP (P240) Antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells, using MBS610766 INCENP (P240) Antibody (blue) compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of mitotic HeLa cells during metaphase (left) or anaphase (right) using MBS610766 INCENP (P240) Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) MBS610766 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-INCENP antibody

INCENP (inner centromere protein antigens 135kD, 155kD) is a chromosomal passenger protein crucial for multiple events that mediate chromosome separation during mitosis (1). At prophase INCENP is associated with chromatin whereas during prometaphase and metaphase it translocates to the inner centromere (1). Depletion of INCENP results in aberrant chromosome alignment at the metaphase plate, incomplete chromosome separation, and disruption of proper spindle formation and cytokinesis (2). INCENP is part of the chromosomal passenger complex that also contains Aurora B, borealin and survivin (2). Aurora B and INCENP are mutually dependent on each other for proper localization (3), and in Drosophila cells and C.elegans embryos that lack INCENP or survivin, Aurora B cannot organize the kinetochores and the midbody (4,5). Phosphorylation on INCENP by CDK1 on Thr59 and Thr388 leads to the association of INCENP with Plk1, another important regulator of mitotic entry and exit (6). Interaction of INCENP with Plk1 is necessary for recruitment of Plk1 to the kinetochores, and the metaphase to anaphase transition (6). Interactions have also been reported between INCENP and heterochromatin protein 1 (HP1) (7) and -tubulin (8).

Product Categories/Family for anti-INCENP antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 102467242
• NCBI GeneID: 3619
• NCBI Accession #: NP_064623.2
• NCBI GenBank Nucleotide #: NM_020238.2
• UniProt Accession #: Q9NQS7; Q5Y192; A8MQD2
• Molecular Weight: 105,429 Da
• NCBI Official Full Name: inner centromere protein isoform 2
• NCBI Official Synonym Full Names: inner centromere protein antigens 135/155kDa
• NCBI Official Symbol: INCENP
• NCBI Official Synonym Symbols: FLJ31633; MGC111393
• NCBI Protein Information: inner centromere protein; OTTHUMP00000236001; OTTHUMP00000236061; chromosomal passenger protein; inner centromere protein INCENP; binds and activates aurora-B and -C in vivo and in vitro
• UniProt Protein Name: Inner centromere protein
• Protein Family: Inner centromere protein
• UniProt Gene Name: INCENP
• UniProt Entry Name: INCE_HUMAN

NCBI Description

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM]

Uniprot Description

INCENP: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. Homodimer or heterodimer. Interacts with H2AFZ. Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with EVI5. Interacts with CDCA8 and BIRC5; interaction is direct. Interacts with CBX5; POGZ and INCENP compete for interaction with CBX5. Interacts with POGZ. Interacts with JTB. Belongs to the INCENP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: kinetochore; centric heterochromatin; lateral element; microtubule; central element; protein complex; spindle; midbody; cytosol; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: mitosis; cytokinesis; mitotic cell cycle; chromosome segregation