MCP2 recombinant protein

Human MCP2 protein, SUMO tag

Cat. Num. AAA9719046

• Gene Names: CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: MCP2; Human MCP2 protein; SUMO tag; CCL; HC14; MCP-2; SCYA8; SCYA10; MCP2 recombinant protein

• Host: E Coli
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4.
• Sequence Positions: 184aa
• Sequence: Gln24-Pro99

• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from human MCP2 (Gln 24-Pro 99) was fused with the SUMO tag at the N-terminus.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human MCP2 protein using the buffer we provided not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Human MCP2 protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig. SDS-PAGE analysis of Human MCP2 protein, SUMO tag.)

Related Product Information for MCP2 recombinant protein

Recombinant Human MCP2 protein, SUMO tag expressed in E Coli.
Background: Chemokine ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a small cytokine belonging to the C-C chemokine family. CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. CCL8's ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.

Product Categories/Family for MCP2 recombinant protein

Cell Culture

NCBI and Uniprot Product Information

• NCBI GI #: 2506280
• NCBI GeneID: 6355
• UniProt Accession #: P80075; P78388; A0AV77
• Molecular Weight: 99
• NCBI Official Full Name: C-C motif chemokine 8
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 8
• NCBI Official Symbol: CCL8
• NCBI Official Synonym Symbols: HC14; MCP2; MCP-2; SCYA8; SCYA10
• NCBI Protein Information: C-C motif chemokine 8; small-inducible cytokine A8; monocyte chemotactic protein 2; monocyte chemoattractant protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)
• UniProt Protein Name: C-C motif chemokine 8
• Protein Family: Meiotic coiled-coil protein
• UniProt Gene Name: CCL8
• UniProt Synonym Gene Names: MCP2; SCYA10; SCYA8; MCP-2
• UniProt Entry Name: CCL8_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; protein kinase activity; phospholipase activator activity

Biological Process: cellular calcium ion homeostasis; positive regulation of catalytic activity; exocytosis; cell-cell signaling; calcium ion transport; response to virus; immune response; chemotaxis; inflammatory response; signal transduction; protein amino acid phosphorylation