Rabbit INCENP Polyclonal Antibody | anti-INCENP antibody

INCENP Polyclonal Antibody

Cat. Num. AAA3006753

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: INCENP; Polyclonal Antibody; INCENP Polyclonal Antibody; Inner centromere protein; anti-INCENP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: INCENP polyclonal antibody detects endogenous levels of INCENP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 918

• Applicable Applications for anti-INCENP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human INCENP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of INCENP polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-INCENP antibody

A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and beta Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex.

NCBI and Uniprot Product Information

• NCBI GI #: 102467235
• NCBI GeneID: 3619
• NCBI Accession #: NP_001035784.1
• NCBI GenBank Nucleotide #: NP_001035784.1
• UniProt Accession #: Q9NQS7
• Molecular Weight: ~ 140kDa
• NCBI Official Full Name: inner centromere protein isoform 1
• NCBI Official Synonym Full Names: inner centromere protein
• NCBI Official Symbol: INCENP
• NCBI Protein Information: inner centromere protein
• UniProt Protein Name: Inner centromere protein
• Protein Family: Inner centromere protein
• UniProt Gene Name: INCENP
• UniProt Entry Name: INCE_HUMAN

NCBI Description

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]