Mouse anti-Human Kindlin-1/KIND1 Monoclonal Antibody | anti-FERMT1 antibody

Kindlin-1/KIND1

Cat. Num. AAA4381677

• Gene Names: FERMT1; URP1; KIND1; DTGCU2; UNC112A; C20orf42
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Kindlin-1/KIND1; Monoclonal Antibody; Fermitin family homolog 1 (Fermt1); Kindlin-1 (KIND1); Kindlerin; Unc-112; UNC112A; URP1; DTGCU2; anti-FERMT1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, lambda
• Clone Number: 4A5.14
• Specificity: Kindlin-1 (also known as KIND1, KINDLERIN, UNC112-RELATED PROTEIN 1 (URP1) is an important component of cell-extracellular matrix adhesion. Kindlin-1 is involved in cell adhesion, possibly via its interaction with integrins and may mediate TGF-beta 1 signaling during tumor progression. Kindlin-1 has been shown to be significantly upregulated in lung and colon carcinomas. Mutations in the kindlin-1 gene, FERMT1, causes Kindler syndrome. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin. We have developed monoclonal anti-kidlin-1 antibodies. This monoclonal antibody recognizes kindlin-1 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 677

• Applicable Applications for anti-FERMT1 antibody: Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IP: 1-2ug per 100-500ug of total protein 1ml of cell lysate; WB: 1-2ug/ml; IHC-P: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes
• Immunogen: Recombinant protein containing Kindlin-1 (residues 216-677)
• Cellular Localization: Cytoplasm
• Hu-chromosome Location: 20p12.3
• Positive Control: 293T whole cell lysate. Human Tonsil or Liver.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)-Formallin

(Formalin-fixed, paraffin-embedded human liver stained with KIND1 Mouse Monoclonal Antibody (4A5.14).)

SDS-Page

(SDS-PAGE Analysis Purified KIND1 Mouse Monoclonal Antibody (4A5.14). Confirmation of Purity and Integrity of Antibody.)

References

Histopathology (2007) 51(4):499-508.

NCBI and Uniprot Product Information

• NCBI GI #: 116686114
• NCBI GeneID: 55612
• NCBI Accession #: NP_060141.3
• NCBI GenBank Nucleotide #: NP_060141.3
• UniProt Accession #: Q9BQL6
• Molecular Weight: 77kDa
• NCBI Official Full Name: fermitin family homolog 1
• NCBI Official Synonym Full Names: fermitin family member 1
• NCBI Official Symbol: FERMT1
• NCBI Official Synonym Symbols: URP1; KIND1; DTGCU2; UNC112A; C20orf42
• NCBI Protein Information: fermitin family homolog 1
• UniProt Protein Name: Fermitin family homolog 1
• Protein Family: Fermitin family
• UniProt Gene Name: FERMT1
• UniProt Synonym Gene Names: C20orf42; KIND1; URP1
• UniProt Entry Name: FERM1_HUMAN

NCBI Description

This gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq, Dec 2009]

Uniprot Description

kindlin-1: Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites. May mediate TGF-beta 1 signaling in tumor progression. Defects in FERMT1 are the cause of Kindler syndrome (KINDS). An autosomal recessive skin disorder characterized by skin blistering, photosensitivity, progressive poikiloderma, and extensive skin atrophy. Additional clinical features include gingival erosions, ocular, esophageal, gastrointestinal and urogenital involvement, and an increased risk of mucocutaneous malignancy. Although most FERMT1 mutations are predicted to lead to premature termination of translation, and to loss of FERMT1 function, significant clinical variability is observed among patients. There is an association of FERMT1 missense and in-frame deletion mutations with milder disease phenotypes, and later onset of complications (PubMed:21936020). Belongs to the kindlin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20p12.3

Cellular Component: filamentous actin; focal adhesion; cell junction; cytosol

Biological Process: keratinocyte proliferation; cell adhesion; keratinocyte migration

Disease: Kindler Syndrome