Rabbit INCENP Polyclonal Antibody | anti-INCENP antibody

INCENP Antibody

Cat. Num. AAA9213784

• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: INCENP; Polyclonal Antibody; INCENP Antibody; Inner centromere protein; anti-INCENP antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
• Concentration: 1mg/ml (varies by lot)
• Sequence Positions: 501-560
• Sequence Length: 914

• Applicable Applications for anti-INCENP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~ 1:1000
• Antigen Type: synthetic peptide
• Antigen Source: KLH conjugated synthetic peptide derived from human INCENP
• Preparation and Storage: Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-INCENP Antibody at 1:1000 dilutionLane 1: Molt-4 whole cell lysatesLane 2: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 105 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-INCENP antibody

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.

References

Adams R.R.,et al.Chromosoma 110:65-74(2001).
Li X.,et al.J. Biol. Chem. 279:47201-47211(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ainsztein A.M.,et al.J. Cell Biol. 143:1763-1774(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 102467235
• NCBI GeneID: 3619
• NCBI Accession #: NP_001035784.1
• NCBI GenBank Nucleotide #: NM_001040694.1
• UniProt Accession #: Q9NQS7; Q5Y192; A8MQD2
• Molecular Weight: 105429
• NCBI Official Full Name: inner centromere protein isoform 1
• NCBI Official Synonym Full Names: inner centromere protein antigens 135/155kDa
• NCBI Official Symbol: INCENP
• NCBI Protein Information: inner centromere protein
• UniProt Protein Name: Inner centromere protein
• Protein Family: Inner centromere protein
• UniProt Gene Name: INCENP
• UniProt Entry Name: INCE_HUMAN

NCBI Description

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]

Uniprot Description

INCENP: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. Homodimer or heterodimer. Interacts with H2AFZ. Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with EVI5. Interacts with CDCA8 and BIRC5; interaction is direct. Interacts with CBX5; POGZ and INCENP compete for interaction with CBX5. Interacts with POGZ. Interacts with JTB. Belongs to the INCENP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: kinetochore; lateral element; centric heterochromatin; microtubule; central element; protein complex; spindle; midbody; cytosol; chromosome, pericentric region; chromocenter

Molecular Function: protein binding

Biological Process: mitosis; cytokinesis; mitotic cell cycle; chromosome segregation