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Western Blot (WB)
(Anti-ILKAP rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa)
Rabbit anti-Human ILKAP Polyclonal Antibody | anti-ILKAP antibody
Anti-ILKAP Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
ILKAP; Polyclonal Antibody; Anti-ILKAP Antibody; Rabbit Polyclonal; ILKAP Antibody; Rabbit PAb; Antigen Affinity Purified; integrin-linked kinase-associated serine/threonine phosphatase; Anti-ILKAP2 Antibody; Anti-ILKAP3 Antibody; Anti-PP2C-DELTA Antibody; anti-ILKAP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ILKAP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ILKAP antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
IHC-P: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
Immunogen
E Coli-derived Human ILKAP fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ILKAP fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ILKAP rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa)
Western Blot (WB)
(Anti-ILKAP rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: NIH-3T3 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human ILKAP in human lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ILKAP in human lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ILKAP in human stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ILKAP in human stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(ILKAP was immunoprecipitated using: Lane A:0.5 mg U-251MG Whole Cell Lysate 4 uL anti-ILKAP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ILKAP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size :45 kDa)
Immunoprecipitation (IP)
(ILKAP was immunoprecipitated using: Lane A:0.5 mg U-251MG Whole Cell Lysate 4 uL anti-ILKAP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ILKAP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size :45 kDa)
Related Product Information for anti-ILKAP antibody
Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is a cytoplasm protein that belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels are evident in various smooth muscle tissues. ILKAP may play a role in the regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin-linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase (ILK) plays key roles in a variety of cell functions, including cell proliferation, adhesion, and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.
Product Categories/Family for anti-ILKAP antibody
References
Leung-Hagesteijn C. et al., 2001, EMBO J. 20: 2160-70. Kumar, A.S. et al., 2004, Oncogene. 23 (19):3454-61. Lammers, T. et al., 2007, Crit Rev Biochem Mol Biol. 42 (6):437-61. Nakrieko, K.A. et al., 2008, Cell Cycle. 7 (14):2157-66.
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Product Notes
The ILKAP (Catalog #AAA8108630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ILKAP Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ILKAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:500-1:2000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the ILKAP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILKAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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