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Western Blot (WB) (Fig. Western Blot analysis of various cells using ILKAP Polyclonal Antibody.)

Rabbit ILKAP Polyclonal Antibody | anti-ILKAP antibody

ILKAP Polyclonal Antibody

Gene Names
ILKAP; PPM1O; ILKAP2; ILKAP3; PP2C-DELTA
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ILKAP; Polyclonal Antibody; ILKAP Polyclonal Antibody; Rabbit Anti-ILKAP Polyclonal Antibody; Integrin-linked kinase-associated serine/threonine phosphatase 2C; anti-ILKAP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
ILKAP Polyclonal Antibody detects endogenous levels of ILKAP protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
392
Applicable Applications for anti-ILKAP antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:20000
Immunogen
Synthesized peptide derived from the internal region of human ILKAP at AA range: 10-90
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig. Western Blot analysis of various cells using ILKAP Polyclonal Antibody.)

Western Blot (WB) (Fig. Western Blot analysis of various cells using ILKAP Polyclonal Antibody.)
Related Product Information for anti-ILKAP antibody
Integrin-linked kinase-associated serine/threonine phosphatase 2C encoded by ILKAP is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
integrin-linked kinase-associated serine/threonine phosphatase 2C
NCBI Official Synonym Full Names
ILK associated serine/threonine phosphatase
NCBI Official Symbol
ILKAP
NCBI Official Synonym Symbols
PPM1O; ILKAP2; ILKAP3; PP2C-DELTA
NCBI Protein Information
integrin-linked kinase-associated serine/threonine phosphatase 2C
UniProt Protein Name
Integrin-linked kinase-associated serine/threonine phosphatase 2C
UniProt Gene Name
ILKAP
UniProt Synonym Gene Names
ILKAP
UniProt Entry Name
ILKAP_HUMAN

NCBI Description

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

ILKAP: a protein serine/threonine phosphatase of the PP2C family. Can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; regulation of DNA replication during S phase

Research Articles on ILKAP

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Product Notes

The ILKAP ilkap (Catalog #AAA9702320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ILKAP Polyclonal Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ILKAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the ILKAP ilkap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILKAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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