ILKAP recombinant protein

ILKAP Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121213

• Gene Names: ILKAP; ILKAP2; ILKAP3; PP2C-DELTA
• Purity: >92% as determined by SDS-PAGE
• Synonyms: ILKAP; ILKAP Protein; Human; Recombinant (His Tag); Human ILKAP Protein (His Tag); ILKAP2 Protein; ILKAP3 Protein; PP2C-DELTA Protein; integrin-linked kinase-associated serine/threonine phosphatase; ILKAP recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His392

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human ILKAP (Q9H0C8)(Met 1-His 392) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ILKAP recombinant protein

Background: Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is a cytoplasm protein that belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels are evident in various smooth muscle tissues. ILKAP may play a role in the regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin-linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase (ILK) plays key roles in a variety of cell functions, including cell proliferation, adhesion, and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.

Product Categories/Family for ILKAP recombinant protein

Phosphatases and Regulators; AKT Signaling Pathway

References

Leung-Hagesteijn C. et al., 2001, EMBO J. 20: 2160-70. Kumar,A.S. et al., 2004, Oncogene. 23 (19):3454-61. Lammers,T. et al., 2007, Crit Rev Biochem Mol Biol. 42 (6):437-61. Nakrieko,K.A. et al., 2008, Cell Cycle. 7 (14):2157-66.

NCBI and Uniprot Product Information

• NCBI GI #: 13540531
• NCBI GeneID: 80895
• NCBI Accession #: NP_110395.1
• NCBI GenBank Nucleotide #: NM_030768.2
• UniProt Accession #: Q9H0C8; B3KM39
• Molecular Weight: 42,907 Da
• NCBI Official Full Name: integrin-linked kinase-associated serine/threonine phosphatase 2C
• NCBI Official Synonym Full Names: integrin-linked kinase-associated serine/threonine phosphatase
• NCBI Official Symbol: ILKAP
• NCBI Official Synonym Symbols: ILKAP2; ILKAP3; PP2C-DELTA
• NCBI Protein Information: integrin-linked kinase-associated serine/threonine phosphatase 2C; protein phosphatase 2c, delta isozyme; integrin-linked kinase associated phosphatase
• UniProt Protein Name: Integrin-linked kinase-associated serine/threonine phosphatase 2C
• Protein Family: Integrin-linked kinase-associated serine/threonine phosphatase
• UniProt Gene Name: ILKAP
• UniProt Synonym Gene Names: ILKAP
• UniProt Entry Name: ILKAP_HUMAN

NCBI Description

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

ILKAP: a protein serine/threonine phosphatase of the PP2C family. Can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; regulation of DNA replication during S phase