Rabbit anti-Human ILKAP Polyclonal Antibody | anti-ILKAP antibody

Anti-ILKAP Antibody, Rabbit Polyclonal

Cat. Num. AAA8106576

• Gene Names: ILKAP; ILKAP2; ILKAP3; PP2C-DELTA
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ILKAP; Polyclonal Antibody; Anti-ILKAP Antibody; Rabbit Polyclonal; ILKAP Antibody; Rabbit PAb; Antigen Affinity Purified; integrin-linked kinase-associated serine/threonine phosphatase; Anti-ILKAP2 Antibody; Anti-ILKAP3 Antibody; Anti-PP2C-DELTA Antibody; anti-ILKAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ILKAP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ILKAP antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human ILKAP protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ILKAP (rh ILKAP). ILKAP specific IgG was purified by Human ILKAP affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ILKAP rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa)

Immunoprecipitation (IP)

(ILKAP was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate2 uL anti-ILKAP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ILKAP rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)

Related Product Information for anti-ILKAP antibody

Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is a cytoplasm protein that belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels are evident in various smooth muscle tissues. ILKAP may play a role in the regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin-linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase (ILK) plays key roles in a variety of cell functions, including cell proliferation, adhesion, and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.

Product Categories/Family for anti-ILKAP antibody

Phosphatases and Regulators; AKT Signaling Pathway

References

Leung-Hagesteijn C. et al., 2001, EMBO J. 20: 2160-70. Kumar, A.S. et al., 2004, Oncogene. 23 (19):3454-61. Lammers, T. et al., 2007, Crit Rev Biochem Mol Biol. 42 (6):437-61. Nakrieko, K.A. et al., 2008, Cell Cycle. 7 (14):2157-66.

NCBI and Uniprot Product Information

• NCBI GI #: 13540531
• NCBI GeneID: 80895
• NCBI Accession #: NP_110395.1
• NCBI GenBank Nucleotide #: NM_030768.2
• UniProt Accession #: Q9H0C8; B3KM39
• Molecular Weight: 42,907 Da
• NCBI Official Full Name: integrin-linked kinase-associated serine/threonine phosphatase 2C
• NCBI Official Synonym Full Names: integrin-linked kinase-associated serine/threonine phosphatase
• NCBI Official Symbol: ILKAP
• NCBI Official Synonym Symbols: ILKAP2; ILKAP3; PP2C-DELTA
• NCBI Protein Information: integrin-linked kinase-associated serine/threonine phosphatase 2C; protein phosphatase 2c, delta isozyme; integrin-linked kinase associated phosphatase
• UniProt Protein Name: Integrin-linked kinase-associated serine/threonine phosphatase 2C
• Protein Family: Integrin-linked kinase-associated serine/threonine phosphatase
• UniProt Gene Name: ILKAP
• UniProt Synonym Gene Names: ILKAP
• UniProt Entry Name: ILKAP_HUMAN

NCBI Description

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

ILKAP: a protein serine/threonine phosphatase of the PP2C family. Can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; regulation of DNA replication during S phase