Western Blot (WB)
(Anti-PPM1G rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: 292T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:80 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PPM1G Polyclonal Antibody | anti-PPM1G antibody
Anti-PPM1G Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 4-6uL/mg of lysate
Western Blot (WB)
(Anti-PPM1G rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: 292T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:80 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PPM1G rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: 292T Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:80 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(PPM1G was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-PPM1G rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPM1G rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size: 72 kDa)
Immunoprecipitation (IP)
(PPM1G was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-PPM1G rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPM1G rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size: 72 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
Uniprot Description
PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
Protein type: Spliceosome; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity
Biological Process: protein amino acid dephosphorylation; cell cycle arrest
Research Articles on PPM1G
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Product Notes
The PPM1G ppm1g (Catalog #AAA8105155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPM1G Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the PPM1G ppm1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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