Western Blot (WB)
(Ikaros pAb tested by Western blot. Detection of Ikaros by Western blot analysis. Ikaros was detected using a 1:1,000 dilution of Ikaros pAb with 20 ug mouse spleen tissue extract.)
Rabbit anti-Mouse Ikaros Polyclonal Antibody | anti-Ikaros antibody
Ikaros antibody (pAb)
Reactivity
Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Gel Super Shift Assay, Western Blot
Synonyms
Ikaros; Polyclonal Antibody; Ikaros antibody (pAb); IKZF1; LyF-1; hlk-1; Zfpn1a1; Znfn1a1; anti-Ikaros antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Ikaros antibody
Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500 dilution
Ikaros may be present as multiple protein isoforms due to alternative splicing. Commonly, the two largest isoforms are detected as seen in the example Western blot image.
WB: 1:500 dilution
Ikaros may be present as multiple protein isoforms due to alternative splicing. Commonly, the two largest isoforms are detected as seen in the example Western blot image.
Immunogen
This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Ikaros pAb tested by Western blot. Detection of Ikaros by Western blot analysis. Ikaros was detected using a 1:1,000 dilution of Ikaros pAb with 20 ug mouse spleen tissue extract.)
Western Blot (WB)
(Ikaros pAb tested by Western blot. Detection of Ikaros by Western blot analysis. Ikaros was detected using a 1:1,000 dilution of Ikaros pAb with 20 ug mouse spleen tissue extract.)
Related Product Information for anti-Ikaros antibody
Short Description: Our Ikaros antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Supershift and Western blot; it has been shown to react with Mouse samples.
Background: Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.
Background: Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.
Product Categories/Family for anti-Ikaros antibody
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62kDa
Protein Family
Similar Products
Product Notes
The Ikaros (Catalog #AAA388444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ikaros antibody (pAb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ikaros can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB). Applications Validated: WB: 1:500 dilution Ikaros may be present as multiple protein isoforms due to alternative splicing. Commonly, the two largest isoforms are detected as seen in the example Western blot image. Researchers should empirically determine the suitability of the Ikaros for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ikaros, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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