Western Blot (WB)
(Western blot analysis of GADD153 expression in Jurkat (A); HeLa (B) whole cell lysates.)
Rabbit GADD153 Polyclonal Antibody | anti-GADD153 antibody
GADD153 Antibody
Gene Names
DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Immunogen Affinity Chromatography
Synonyms
GADD153; Polyclonal Antibody; GADD153 Antibody; Purified Polyclonal GADD153 Antibody; Polyclonal GADD153 antibody; Anti-GADD153 antibody; CHOP antibody; CHOP10 antibody; GADD153 antibody; DNA damage-inducible transcript 3 protein antibody; DDIT-3 antibody; C/EBP zeta antibody; C/EBP-homologous protein antibody; C/EBP-homologous protein 10 antibody; CHOP-10 antibody; CCAAT/enhancer-binding protein homologous protein antibody; Growth arrest and DNA damage-inducible protein GADD153 antibody; anti-GADD153 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GADD153 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
168
Applicable Applications for anti-GADD153 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
IP: 1:10-1:100
IHC: 1:100-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
IP: 1:10-1:100
Immunogen
GADD153 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GADD153 as the immunogen
Cross-Reactivity
Monkey, Pig
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of GADD153 expression in Jurkat (A); HeLa (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GADD153 expression in Jurkat (A); HeLa (B) whole cell lysates.)
Related Product Information for anti-GADD153 antibody
GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein.
Product Categories/Family for anti-GADD153 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Gadd153
NCBI Official Synonym Full Names
DNA damage inducible transcript 3
NCBI Official Symbol
DDIT3
NCBI Official Synonym Symbols
CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta
NCBI Protein Information
DNA damage-inducible transcript 3 protein
NCBI Description
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
Research Articles on GADD153
Similar Products
Product Notes
The GADD153 (Catalog #AAA5316136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GADD153 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:100-1:200 IF: 1:100-1:500 ICC: 1:100-1:500 IP: 1:10-1:100. Researchers should empirically determine the suitability of the GADD153 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GADD153, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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