Chromatin Immunoprecipitation (ChIP)
(RBPJ antibody (mAb) tested by ChIP. Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG.)
Rat anti-Human, Mouse RBPJ Monoclonal Antibody | anti-RBPJ antibody
RBPJ antibody (mAb)(Clone 1F1)
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Gel Super Shift Assay
Purity
Protein G Chromatography
Synonyms
RBPJ; Monoclonal Antibody; RBPJ antibody (mAb)(Clone 1F1); SUH; csl; AOS3; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1; anti-RBPJ antibody
Host
Rat
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1F1
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-RBPJ antibody
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Electrophoretic Mobility Shift Assay (EMSA)
Application Notes
Applications Validated:
ICC/IF: 0.5ug/ml dilution
ICC/IF: 0.5ug/ml dilution
Immunogen
This antibody was raised against a full-length recombinant protein corresponding to human RBPJ.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(RBPJ antibody (mAb) tested by ChIP. Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG.)
Chromatin Immunoprecipitation (ChIP)
(RBPJ antibody (mAb) tested by ChIP. Chromatin IP was performed using chromatin of an Epstein-Barr virus infected lymphoblastoid cell line ( 2 x 10^6 cell eqivalents per ChIP) and RBPJ antibody or the equivalent amount of IgG negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs for a positive and negative control region. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG.)
Immunofluorescence (IF)
(RBPJ antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with RBPJ antibody at a 0.5 ug/ml dilution.)
Immunofluorescence (IF)
(RBPJ antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with RBPJ antibody at a 0.5 ug/ml dilution.)
Related Product Information for anti-RBPJ antibody
Short Description: Our RBPJ antibody (mAb)(Clone 1F1) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence and Supershift; it has been shown to react with Human and Mouse samples.
Background: RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.
Background: RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.
Product Categories/Family for anti-RBPJ antibody
NCBI and Uniprot Product Information
Molecular Weight
68kDa
Protein Family
Similar Products
Product Notes
The RBPJ (Catalog #AAA388927) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RBPJ antibody (mAb)(Clone 1F1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RBPJ can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Electrophoretic Mobility Shift Assay (EMSA). Applications Validated: ICC/IF: 0.5ug/ml dilution. Researchers should empirically determine the suitability of the RBPJ for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBPJ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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