Rabbit anti-Human, Mouse IFN Beta / Interferon Beta Polyclonal Antibody | anti-IFNB1 antibody

Rabbit Polyclonal (IgG) to Human IFN Beta / Interferon Beta

Cat. Num. AAA241277

• Gene Names: IFNB1; IFB; IFF; IFNB
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: IFN Beta / Interferon Beta; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human IFN Beta / Interferon Beta; Anti-IFN Beta / Interferon Beta Antibody (Internal) IHC-plus; IFF; IFB; IFNB; Interferon beta; Interferon; beta 1; fibroblast; Fibroblast interferon; IFN-beta; IFN Beta; Human IFN Beta; Interferon Beta; anti-IFNB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: An 17 amino acid peptide from near the center of humAn IFN-b
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 187

• Applicable Applications for anti-IFNB1 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin, Immunofluorescence (IF), Western Blot (WB).
• Application Notes: Immunohiostochemistry: MBS241277 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE), human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody slides were incubated with iotinylated secondary antiobdy, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a phatologist to confirm staining specifity. The optimal working concentration MBS241277 was determined to be 5 ug/mL.; ; ; Immunohistochemistry-Paraffin; Western Blot (WB).; ; Optimal dilution to be determined by the researcher.
• Target: IFN Beta/ Interferon Beta
• Immunogen Species: IFN Beta/Interferon Beta antibody was raised against Human.
• Epitope: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Interferon Beta antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of IFN-beta in Human Liver cells with IFN-beta antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of IFN-b in A-20 cell lysate with IFN-b antibody at 5 ug/ml.)

Related Product Information for anti-IFNB1 antibody

Interferon beta (IFNB1), acting via STAT1 and STAT2, upregulates and downregulates a wide variety of genes, most of which are involved in the antiviral immune response. Most IFN responses are induced by the presence of dsRNA, but both DNA and RNA viruses are sensitive to the effects of IFN-beta.

Product Categories/Family for anti-IFNB1 antibody

Family: Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 4504603
• NCBI GeneID: 3456
• NCBI Accession #: NP_002167.1
• NCBI GenBank Nucleotide #: NM_002176.2
• NCBI Official Full Name: interferon beta
• NCBI Official Synonym Full Names: interferon, beta 1, fibroblast
• NCBI Official Symbol: IFNB1
• NCBI Official Synonym Symbols: IFB; IFF; IFNB
• NCBI Protein Information: interferon beta; IFN-beta; fibroblast interferon
• UniProt Protein Name: Interferon beta
• Protein Family: Interferon
• UniProt Gene Name: IFNB1
• UniProt Synonym Gene Names: IFB; IFNB; IFN-beta
• UniProt Entry Name: IFNB_HUMAN

Uniprot Description

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response