OXTR / Oxytocin Receptor Polyclonal Antibody | anti-OXTR antibody

Rabbit Polyclonal to Human OXTR / Oxytocin Receptor

Cat. Num. AAA242900

• Gene Names: OXTR; OT-R
• Reactivity: Human; Predicted Reactivity: Monkey
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: OXTR / Oxytocin Receptor; Polyclonal Antibody; Rabbit Polyclonal to Human OXTR / Oxytocin Receptor; Anti-OXTR / Oxytocin Receptor Antibody (Cytoplasmic Domain) IHC-plus; OXTR; Oxytocin receptor; Oxt-r; OT-R; Human OXTR; Oxytocin Receptor; anti-OXTR antibody

• Host: OXTR / Oxytocin Receptor antibody was produced in Rabbit
• Reactivity: Human; Predicted Reactivity: Monkey
• Clonality: Polyclonal
• Specificity: Human Oxytocin Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, less than 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 389

• Applicable Applications for anti-OXTR antibody: Immunohistochemistry, Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (10 ug/ml); ; Optimal dilution to be determined by the researcher.
• Target: OXTR/ Oxytocin Receptor
• Family/Subfamily: GPCR/Vasopressin/oxytocin
• Antigen Type: Synthetic peptide
• Immunogen Species: OXTR/Oxytocin Receptor antibody was raised against Human.
• Immunogen: OXTR/Oxytocin Receptor antibody was raised against synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Oxytocin Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (94%).
• Immunogen: OXTR / Oxytocin Receptor antibody was raised against synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Oxytocin Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (94%).
• Epitope: Cytoplasmic Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Aliquot and store undiluted at -20°C or below up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-OXTR / Oxytocin Receptor antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-Oxytocin Receptor antibody IHC of human uterus, term. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-OXTR antibody

The oxytocin receptor belongs to the vasopressin/oxytocin family of GPCRs because it binds similar cyclic nonapeptide hormones. The oxytocin receptor functions primarily during labor and lactation. There is a dramatic increase in the number of oxytocin receptors just before the onset of labor causing the uterine muscles to become exceedingly sensitive to oxytocin.

Product Categories/Family for anti-OXTR antibody

Family: GPCR; Subfamily: Vasopressin/oxytocin

NCBI and Uniprot Product Information

• NCBI GI #: 32307152
• NCBI GeneID: 5021
• NCBI Accession #: NP_000907.2
• NCBI GenBank Nucleotide #: NM_000916.3
• UniProt Accession #: P30559; Q15071
• NCBI Official Full Name: oxytocin receptor
• NCBI Official Synonym Full Names: oxytocin receptor
• NCBI Official Symbol: OXTR
• NCBI Official Synonym Symbols: OT-R
• NCBI Protein Information: oxytocin receptor
• UniProt Protein Name: Oxytocin receptor
• Protein Family: Oxytocin receptor
• UniProt Gene Name: OXTR
• UniProt Synonym Gene Names: OT-R
• UniProt Entry Name: OXYR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition. [provided by RefSeq, Jul 2008]

Uniprot Description

OXTR: Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: cell-cell adherens junction; microvillus; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: oxytocin receptor activity; peptide hormone binding; peptide binding; vasopressin receptor activity

Biological Process: lactation; response to peptide hormone stimulus; response to anoxia; heart development; female pregnancy; positive regulation of synaptic transmission, glutamatergic; response to estradiol stimulus; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; muscle contraction; response to drug; maternal behavior; eating behavior; response to amphetamine; positive regulation of synaptic transmission, GABAergic; social behavior; sleep; response to cocaine; memory; cellular response to hormone stimulus; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; telencephalon development; suckling behavior; gut development; regulation of systemic arterial blood pressure by vasopressin; response to cytokine stimulus; maternal process involved in parturition; positive regulation of vasoconstriction; sperm ejaculation; response to progesterone stimulus; positive regulation of blood pressure