Rabbit anti-Human IFIT3 Polyclonal Antibody | anti-IFIT3 antibody

IFIT3 (Interferon-induced Protein with Tetratricopeptide Repeats 3, IFIT-3, CIG49, ISG-60, Interferon-induced 60kD Protein, IFI-60K, Interferon-induced Protein with Tetratricopeptide Repeats 4, IFIT-4, Retinoic Acid-induced Gene G-Protein, P60, RIG-G, CIG

Cat. Num. AAA6382157

• Gene Names: IFIT3; P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; cig41; CIG-49; GARG-49
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IFIT3; Polyclonal Antibody; IFIT3 (Interferon-induced Protein with Tetratricopeptide Repeats 3; IFIT-3; CIG49; ISG-60; Interferon-induced 60kD Protein; IFI-60K; Interferon-induced Protein with Tetratricopeptide Repeats 4; IFIT-4; Retinoic Acid-induced Gene G-Protein; P60; RIG-G; CIG; anti-IFIT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IFIT3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-IFIT3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human IFIT3, aa1-490 (NP_001540.2).
• Immunogen Sequence: MSEVTKNSLEKILPQLKCHFTWNLFKEDSVSRDLEDRVCNQIEFLNTEFKATMYNLLAYIKHLDGNNEAALECLRQAEELIQQEHADQAEIRSLVTWGNYAWVYYHLGRLSDAQIYVDKVKQTCKKFSNPYSIEYSELDCEEGWTQLKCGRNERAKVCFEKALEEKPNNPEFSSGLAIAMYHLDNHPEKQFSTDVLKQAIELSPDNQYVKVLLGLKLQKMNKEAEGEQFVEEALEKSPCQTDVLRSAAKFYRRKGDLDKAIELFQRVLESTPNNGYLYHQIGCCYKAKVRQMQNTGESEASGNKEMIEALKQYAMDYSNKALEKGLNPLNAYSDLAEFLETECYQTPFNKEVPDAEKQQSHQRYCNLQKYNGKSEDTAVQHGLEGLSISKKSTDKEEIKDQPQNVSENLLPQNAPNYWYLQGLIHKQNGDLLQAAKCYEKELGRLLRDAPSGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQLN
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(IFIT3 rabbit polyclonal antibody. Western Blot analysis of IFIT3 expression in human spleen.)

Western Blot (WB)

(Western Blot analysis of IFIT3 expression in transfected 293T cell line by IFIT3 polyclonal antibody. Lane 1: IFIT3 transfected lysate (56kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-IFIT3 antibody

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

Product Categories/Family for anti-IFIT3 antibody

Antibodies; Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 31542980
• NCBI GeneID: 3437
• NCBI Accession #: NP_001540.2
• NCBI GenBank Nucleotide #: NM_001549
• UniProt Accession #: O14879
• Molecular Weight: 53 kDa
• NCBI Official Full Name: interferon-induced protein with tetratricopeptide repeats 3 isoform a
• NCBI Official Synonym Full Names: interferon induced protein with tetratricopeptide repeats 3
• NCBI Official Symbol: IFIT3
• NCBI Official Synonym Symbols: P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; cig41; CIG-49; GARG-49
• NCBI Protein Information: interferon-induced protein with tetratricopeptide repeats 3
• UniProt Protein Name: Interferon-induced protein with tetratricopeptide repeats 3
• Protein Family: Interferon-induced protein with tetratricopeptide repeats
• UniProt Gene Name: IFIT3
• UniProt Synonym Gene Names: CIG-49; IFI60; IFIT4; ISG60; IFIT-3; IFI-60K; IFIT-4; P60; RIG-G
• UniProt Entry Name: IFIT3_HUMAN

Uniprot Description

IFIT3: IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS- mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin- dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2. Belongs to the IFIT family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: negative regulation of cell proliferation; cytokine and chemokine mediated signaling pathway; response to virus; defense response to virus; negative regulation of apoptosis