GH1 active protein

Recombinant Mouse GH1

Cat. Num. AAA4159923

• Gene Names: Gh; Gh1; Ghb1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GH1; Recombinant Mouse GH1; Somatotropin; Growth hormone; Gh1; Gh; Recombinant Mouse Growth Hormone (GH1); GH1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from 0.2 um filtered solution in Tris and NaCl
• Sequence Length: 216

• Bioactivity: The ED50 was determined by the dose-dependent proliferation assay using the mouse myoblast cell line and was to be <0.5ng/ml.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for GH1 active protein

The physiological activity for which growth hormone is best known is the promotion of growth of bone, cartilage, and soft tissues. Detectable levels of growth hormone are found throughout the remainder of adulthood, suggesting other functions in addition to promotion of growth. Growth hormone may be important for the maintenance of lean body mass; most growth-promoting effects of growth hormone are mediated by IGF-1 the synthesis of which is regulated by growth hormone. The biological activities of growth hormone are mediated by receptors belonging to one of the Cytokine receptor families. Growth hormone has been shown to be produced by T cells, B cells, and macrophages. In human lymphocytes growth hormone appears to up-regulate its own expression. Growth hormone appears to act as an enhancer of immune responses and is produced in considerable amounts by T helper cells.

NCBI and Uniprot Product Information

• NCBI GI #: 6679997
• NCBI GeneID: 14599
• NCBI Accession #: NP_032143.1
• NCBI GenBank Nucleotide #: NP_032143.1
• UniProt Accession #: P06880
• Molecular Weight: 22.0 kDa
• NCBI Official Full Name: somatotropin
• NCBI Official Synonym Full Names: growth hormone
• NCBI Official Symbol: Gh
• NCBI Official Synonym Symbols: Gh1; Ghb1
• NCBI Protein Information: somatotropin
• UniProt Protein Name: Somatotropin
• Protein Family: Somatotropin
• UniProt Gene Name: Gh1
• UniProt Synonym Gene Names: Gh
• UniProt Entry Name: SOMA_MOUSE

Uniprot Description

GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Hormone

Cellular Component: extracellular space; mitochondrion; extracellular region; plasma membrane; trans-Golgi network; cytosol; nucleus; secretory granule

Molecular Function: growth hormone receptor binding; metal ion binding; hormone activity

Biological Process: response to food; response to light stimulus; positive regulation of neurogenesis; cellular response to insulin stimulus; neuroblast proliferation; positive regulation of multicellular organism growth; glucose transport; regulation of steroid hormone receptor signaling pathway; signal transduction; alveolus development; positive regulation of growth