Typical Testing Data/Standard Curve (for reference only)
General Indole 3 Acetic Acid (IAA) CLIA Kit | IAA clia kit
General Indole 3 Acetic Acid (IAA) CLIA Kit
Reactivity
General
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Indole 3 Acetic Acid (IAA); General Indole 3 Acetic Acid (IAA) CLIA Kit; Indole 3 Acetic Acid; IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin; IAA clia kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of Indole 3 Acetic Acid (IAA).
No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.
No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.
Sequence Length
825
Applicable Applications for IAA clia kit
Chemiluminescence Immunoassay (CLIA)
Assay Type
Competitive Inhibition
Samples
Tissue or cell culture supernates
Detection Range
1.56-400ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.59ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Indole 3 Acetic Acid (IAA). A competitive inhibition reaction is launched between biotin labeled Indole 3 Acetic Acid (IAA) and unlabeled Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Indole 3 Acetic Acid (IAA) level in the sample or standard.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
indole-3-acetic acid
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Product Notes
The General IAA (Catalog #AAA2707923) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707923 CLIA Kit recognizes General IAA. AAA Biotech's Indole 3 Acetic Acid (IAA) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the IAA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Indole 3 Acetic Acid (IAA), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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