Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit | GABARAPL2 elisa kit

Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit

Cat. Num. AAA2706647

• Gene Names: GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
• Reactivity: Human
• Synonyms: GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2); Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit; ATG8; GATE16; GEF2; FLC3A; General protein transport factor p16; Ganglioside expression factor 2; Golgi-associated ATPase enhancer of 16 kDa; MAP1 light chain 3-related; GABARAPL2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2).; No significant cross-reactivity or interference between GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) and analogues was observed.
• Sequence Length: 117

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GABARAPL2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13375573
• NCBI GeneID: 11345
• Molecular Weight: 13,667 Da
• NCBI Official Full Name: GABA-A receptor-associated protein like 2
• NCBI Official Synonym Full Names: GABA type A receptor associated protein like 2
• NCBI Official Symbol: GABARAPL2
• NCBI Official Synonym Symbols: ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 2
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 2
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAPL2
• UniProt Synonym Gene Names: FLC3A; GEF2; GEF-2; GATE-16

Uniprot Description

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.