Rabbit HSP90beta Polyclonal Antibody | anti-HSP90beta antibody

HSP90beta polyclonal antibody

Cat. Num. AAA565743

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human, Mouse, Sheep, Dog, Pig, Bovine, Guinea pig, Hamster, Rabbit
• Applications: Western Blot
• Purity: Protein A affinity purified.
• Synonyms: HSP90beta; Polyclonal Antibody; HSP90beta polyclonal antibody; HSP86; Heat shock protein 90; pAb; anti-HSP90beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Sheep, Dog, Pig, Bovine, Guinea pig, Hamster, Rabbit
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, bovine, dog, guinea pig, hamster, pig, rabbit, and sheep HSP90beta. Detects a band of ~90kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 724

• Applicable Applications for anti-HSP90beta antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1'000, colorimetric).; Detects a band of ~90kDa by Western blot.
• Immunogen: Recombinant human HSP90beta.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSP90 beta pAb: Lane 1: MW marker, Lane 2: HeLa Cell Lysate, Heat Shocked, Lane 3: HSP90 Native Human Protein, Lane 4: HSP90 alpha Recombinant Human Protein, Lane 5: HSP90 beta Recombinant Human Protein.)

Related Product Information for anti-HSP90beta antibody

The 90 kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells express inducible Hsp90 alpha and constitutive Hsp90 beta isoforms that are encoded by separate genes. The amino acid sequences of human and yeast Hsp90 alpha are 85% and 90% homologous to those of Hsp90 beta, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular protein in most cells. Hsp90 functions as part of the cell's powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signaling and cell cycle control by serving as a chaperone for many key signaling molecules including steroid receptors, cell cycle kinases involved in signal transduction, and p53. As many of these client proteins are known oncogenes, Hsp90 inhibitors such as 17-AAG, a geldanamycin analog, have been of benefit in the treatment of many cancers.

Product Categories/Family for anti-HSP90beta antibody

Protein Synthesis & Folding; Proteolysis & Protein Degradation; Cell Death

NCBI and Uniprot Product Information

• NCBI GI #: 17865718
• NCBI GeneID: 3326
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: Heat shock protein HSP 90-beta
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84

NCBI Description

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Uniprot Description

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; nucleus; signalosome

Molecular Function: double-stranded RNA binding; heat shock protein binding; histone deacetylase binding; kinase binding; nitric-oxide synthase regulator activity; protein binding; protein dimerization activity; TPR domain binding

Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of phosphoprotein phosphatase activity; positive regulation of transforming growth factor beta receptor signaling pathway; protein stabilization; regulation of protein ubiquitination; virion attachment to host cell surface receptor