Mouse HSP90 beta Monoclonal Antibody | anti-HSP86; Heat shock protein 90 beta antibody

HSP90 beta monoclonal antibody (K3705)

Cat. Num. AAA565009

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Porcine, Sheep
• Applications: Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation purified.
• Synonyms: HSP90 beta; Monoclonal Antibody; HSP90 beta monoclonal antibody (K3705); anti-HSP86; Heat shock protein 90 beta antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Porcine, Sheep
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: K3705
• Specificity: Recognizes human, mouse, rat, bovine, dog, chicken, hamster, guinea pig, pig and sheep HSP90beta. Detects a band of ~90kDa by Western blot.
• Purity/Purification: Ammonium sulfate precipitation purified.
• Form/Format: Liquid. In saline, pH 6.9, containing 0.1% sodium azide.
• Sequence Length: 724

• Applicable Applications for anti-HSP86; Heat shock protein 90 beta antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Immunohistochemistry (1:50); Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~90kDa by Western blot.
• Immunogen: Recombinant human HSP90 beta .
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSP90 beta : Lane 1: MW marker, Lane 2: HSP90 Native Human Protein, Lane 3: HSP90 alpha Recombinant Human Protein, Lane 4: HSP90 beta Recombinant Human Protein, Lane 5: HeLa, Lane 6: PC-12, Lane 7: CHO-K1, Lane 8: 3T3.)

Related Product Information for anti-HSP86; Heat shock protein 90 beta antibody

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Product Categories/Family for anti-HSP86; Heat shock protein 90 beta antibody

Protein Synthesis & Folding; Proteolysis & Protein Degradation; Cell Death

NCBI and Uniprot Product Information

• NCBI GI #: 17865718
• NCBI GeneID: 3326
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: Heat shock protein HSP 90-beta
• NCBI Official Synonym Full Names: heat shock protein 90kDa alpha family class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84
• UniProt Entry Name: HS90B_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Uniprot Description

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: apical plasma membrane; basolateral plasma membrane; brush border membrane; cell surface; cytoplasm; cytosol; inclusion body; melanosome; membrane; mitochondrion; nucleoplasm; signalosome

Molecular Function: ATP binding; dATP binding; double-stranded RNA binding; GTP binding; histone deacetylase binding; kinase binding; nitric-oxide synthase regulator activity; protein binding; protein kinase binding; sulfonylurea receptor binding; TPR domain binding; unfolded protein binding

Biological Process: axon guidance; innate immune response; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; placenta development; positive regulation of cell size; positive regulation of nitric oxide biosynthetic process; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; protein folding; regulation of protein ubiquitination; response to salt stress; response to unfolded protein; virion attachment to host cell surface receptor