Rabbit HSP90AB1 Polyclonal Antibody | anti-HSP90AB1 antibody

Anti-HSP90AB1 Antibody

Cat. Num. AAA419529

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: HSP90AB1; Polyclonal Antibody; Anti-HSP90AB1 Antibody; anti-HSP90AB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using HSP90AB1 antibody. Immunohistochemistry of paraffin-embedded human breast cancer using HSP90AB1 antibody at dilution of 1:500 (200x lens). Immunohistochemistry of paraffin-embedded rat pancreas using HSP90AB1 antibody at dilution of 1:500 (200x lens). Immunohistochemistry of paraffin-embedded mouse kidney using HSP90AB1 antibody at dilution of 1:500 (200x lens). Immunofluorescence analysis of U2OS cell using HSP90AB1 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 714

• Applicable Applications for anti-HSP90AB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human HSP90AB1
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-HSP90AB1 antibody

HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 (2, 3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1, 4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1, 3). They also play a role in vesicle formation and protein trafficking (2).

NCBI and Uniprot Product Information

• NCBI GI #: 431822408
• NCBI GeneID: 3326
• NCBI Accession #: NP_001258901.1
• NCBI GenBank Nucleotide #: NM_001271972.1
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: heat shock protein HSP 90-beta isoform c
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84

NCBI Description

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Uniprot Description

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; nucleus; signalosome

Molecular Function: double-stranded RNA binding; heat shock protein binding; histone deacetylase binding; kinase binding; nitric-oxide synthase regulator activity; protein binding; protein dimerization activity; TPR domain binding

Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of phosphoprotein phosphatase activity; positive regulation of transforming growth factor beta receptor signaling pathway; protein stabilization; regulation of protein ubiquitination; virion attachment to host cell surface receptor