Mouse HSP90beta Monoclonal Antibody | anti-HSP90beta antibody

HSP90beta monoclonal antibody (K3701)

Cat. Num. AAA565742

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human, Mouse, Rat, Sheep, Dog, Pig, Bovine, Guinea pig, Hamster, Rabbit
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Ammonium sulfate precipitation purified.
• Synonyms: HSP90beta; Monoclonal Antibody; HSP90beta monoclonal antibody (K3701); HSP84; Heat shock protein 90beta; mAb (K3701); anti-HSP90beta antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Sheep, Dog, Pig, Bovine, Guinea pig, Hamster, Rabbit
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: K3701
• Specificity: Recognizes human, mouse, rat, bovine, dog, guinea pig, hamster, pig, rabbit and sheep HSP90beta. Detects a band of ~90kDa by Western blot.
• Purity/Purification: Ammonium sulfate precipitation purified.
• Form/Format: Liquid. In saline, pH 6.9, containing 0.1% sodium azide.
• Sequence Length: 724

• Applicable Applications for anti-HSP90beta antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Flow Cytometry (10ug/ml), Western Blot (1:1'000, colorimetric).; Detects a band of ~90kDa by Western blot.
• Immunogen: Recombinant human HSP90beta.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis: Lane 1: MW marker, Lane 2: HeL a, Lane 3: PC-12, Lane 4: CHO-K1, Lane 5: HSP90 Native Protein, Lane 6: HSP90 alpha Recombinant Protein (negative control), probed with HSP90 beta mAb (K3701))

Flow Cytometry (FC/FACS)

(Flow cytometric analysis human colon cancer Caco-2 cells analyzed by flow cytometry using isotype control antibody (left) or Hsp90 beta mAb (K3701) (right).)

Related Product Information for anti-HSP90beta antibody

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Product Categories/Family for anti-HSP90beta antibody

Protein Synthesis & Folding; Proteolysis & Protein Degradation; Cell Death

NCBI and Uniprot Product Information

• NCBI GI #: 17865718
• NCBI GeneID: 3326
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: Heat shock protein HSP 90-beta
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84

NCBI Description

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Uniprot Description

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; nucleus; signalosome

Molecular Function: double-stranded RNA binding; heat shock protein binding; histone deacetylase binding; kinase binding; nitric-oxide synthase regulator activity; protein binding; protein dimerization activity; TPR domain binding

Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of phosphoprotein phosphatase activity; positive regulation of transforming growth factor beta receptor signaling pathway; protein stabilization; regulation of protein ubiquitination; virion attachment to host cell surface receptor