HRP Conjugated Goat Anti-human IgA Secondary Antibody
HRP Conjugated Goat Anti-human IgA (alpha-chain specific) secondary antibody
Applications
Dot Blot, Western Blot, ELISA
Synonyms
HRP Conjugated Goat Anti-human IgA; Polyclonal Secondary Antibody; HRP Conjugated Goat Anti-human IgA (alpha-chain specific) secondary antibody; HRP Conjugated Goat Anti-human IgA secondary antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgA
Specificity
This peroxidase conjugated antibody is specific for human IgA and shows no cross-reactivity with human IgGlIgM.
Form/Format
Concentrated, Liquid
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for HRP Conjugated Goat Anti-human IgA secondary antibody
Dot Blot (DB), Western Blot (WB), ELISA (EIA)
Application Notes
Dot blot (Enhanced chemiluminescent coloration): 1:2000-4000
WB (DAB coloration): 1:300-1000
WB (Enhanced chemiluminescent coloration): 1:2000-4000
ELISA (TMB coloration): 1 :20000-40000
Optimal working dilutions must be determined by end users.
WB (DAB coloration): 1:300-1000
WB (Enhanced chemiluminescent coloration): 1:2000-4000
ELISA (TMB coloration): 1 :20000-40000
Optimal working dilutions must be determined by end users.
Immunogen
Human IgA (whole molecule).
Contents
0.5 mg of peroxidase conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.
Labeling Method
Goat anti-humnn IgA is conjugated to ptOTOxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
Preparation of Diluent Buffer
Use 0.01 M TBS or 0.01 M PBS to dilute. See "Recommended Dilutions" below for details.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total Volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01 M TBS: Add 1.2g Tris, 8.5g NaCI; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H20 and adjust pH to 7.2-7.6. Finally, adjust the total Volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HP04 and 0.2g NaH2P04 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation and Storage
At 4 degree C for one year.
Related Product Information for HRP Conjugated Goat Anti-human IgA secondary antibody
Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for human IgA.
Similar Products
Product Notes
The HRP Conjugated Goat Anti-human IgA (Catalog #AAA176676) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HRP Conjugated Goat Anti-human IgA can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB), ELISA (EIA). Dot blot (Enhanced chemiluminescent coloration): 1:2000-4000 WB (DAB coloration): 1:300-1000 WB (Enhanced chemiluminescent coloration): 1:2000-4000 ELISA (TMB coloration): 1 :20000-40000 Optimal working dilutions must be determined by end users. Researchers should empirically determine the suitability of the HRP Conjugated Goat Anti-human IgA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRP Conjugated Goat Anti-human IgA, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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