Rabbit anti-Human Creatine kinase B Polyclonal Antibody | anti-CKB antibody

Anti-Creatine kinase B Antibody, Rabbit Polyclonal

Cat. Num. AAA8108238

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Creatine kinase B; Polyclonal Antibody; Anti-Creatine kinase B Antibody; Rabbit Polyclonal; Creatine kinase B Antibody; Rabbit PAb; Antigen Affinity Purified; creatine kinase; brain; Anti-B-CK Antibody; Anti-BCK Antibody; Anti-CKBB Antibody; Anti-HEL-211 Antibody; Anti-HEL-S-29 Antibody; anti-CKB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Creatine kinase B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CKB antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:10,000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human Creatine kinase B fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human Creatine kinase B fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Creatine kinase B rabbit polyclonal antibody at 1:2000 dilution Lane A: 293T Whole Cell Lysate Lane B: SHSY5Y Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:45 kDa)

Immunoprecipitation (IP)

(CKB was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 4 uL anti-CKB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CKB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size :45 kDa)

Related Product Information for anti-CKB antibody

CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.

References

Wienker TF, et al. (1985) A dominant mutation causing ectopic expression of the creatine kinase B gene maps on chromosome 14. Cytogenet Cell Genet. 40:776. Mariman EC, et al. (1989) Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17(15):6385. Bong S, et al. (2008) Structural studies of human brain-type creatine kinase complexed with the ADP-Mg2+-NO3--creatine transition-state analogue complex. FEBS Letters. 582(28): 3959-65.