Chromatin Immunoprecipitation (ChIP)
(Histone H3 phospho Thr11 pAb tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from the human H9 embryonic stem cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify H3T11ph occupancy. The image on the left shows H3T11ph binding at transcriptional start sites across a 120,000 bp region on chromosome 1. The image on the right is zoomed in to show H3T11ph binding at a single promoter)
Rabbit Histone H3T11ph Polyclonal Antibody | anti-H3T11ph antibody
Histone H3T11ph antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Synonyms
Histone H3T11ph; Polyclonal Antibody; Histone H3T11ph antibody (pAb); anti-H3T11ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version of this antibody that was purified by Protein A Chromatography is also available.
Applicable Applications for anti-H3T11ph antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 5ul per ChIP
ChIP-Seq: 5ul each
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ChIP: 5ul per ChIP
ChIP-Seq: 5ul each
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Conjugation
Phosphorylated
Immunogen
This Histone H3 phospho Thr11 antibody was raised against a peptide including phospho-threonine 11 of histone H3.
Abs Residue
Thr
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3 phospho Thr11 pAb tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from the human H9 embryonic stem cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify H3T11ph occupancy. The image on the left shows H3T11ph binding at transcriptional start sites across a 120,000 bp region on chromosome 1. The image on the right is zoomed in to show H3T11ph binding at a single promoter)
Chromatin Immunoprecipitation (ChIP)
(Histone H3 phospho Thr11 pAb tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from the human H9 embryonic stem cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 22 million sequence tags were mapped to identify H3T11ph occupancy. The image on the left shows H3T11ph binding at transcriptional start sites across a 120,000 bp region on chromosome 1. The image on the right is zoomed in to show H3T11ph binding at a single promoter)
Western Blot (WB)
(.Histone H3 phospho Thr11 pAb tested by Western blot. Western blot probed with Histone H3 phospho Thr11 pAb (1:2,000 dilution). Lane 1: 200 ng recombinant histone H3. Lane 2: 5 ug acid extract of HeLa cells. Lane 3: 5 ug acid extract of HeLa cells treated with colcemid.)
Western Blot (WB)
(.Histone H3 phospho Thr11 pAb tested by Western blot. Western blot probed with Histone H3 phospho Thr11 pAb (1:2,000 dilution). Lane 1: 200 ng recombinant histone H3. Lane 2: 5 ug acid extract of HeLa cells. Lane 3: 5 ug acid extract of HeLa cells treated with colcemid.)
Dot Blot (DB)
(Histone H3 phospho Thr11 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 1:10,000. Lane 1: Peptide phosphorylated at threonine 3. Lane 2: Unmodified threonine 3 peptide. Lane 3: Peptide phosphorylated at threonine 11. Lane 4: Unmodified threonine 11 peptide. Lane 5: Peptide phosphorylated at threonine 45. Lane 6: Unmodified threonine 45 peptide.)
Dot Blot (DB)
(Histone H3 phospho Thr11 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr11 pAb for phospho-Thr11 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 1:10,000. Lane 1: Peptide phosphorylated at threonine 3. Lane 2: Unmodified threonine 3 peptide. Lane 3: Peptide phosphorylated at threonine 11. Lane 4: Unmodified threonine 11 peptide. Lane 5: Peptide phosphorylated at threonine 45. Lane 6: Unmodified threonine 45 peptide.)
Related Product Information for anti-H3T11ph antibody
Short Description: Our Histone H3T11ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Phosphorylation of several residues of histone H3, such as Ser10 (Histone H3 phospho Ser10), Ser28 (Histone H3 phospho Ser28) and Thr11, is tightly correlated with chromosome condensation during both mitosis and meiosis. Localization of histone H3 phospho Thr11 is different in mammals and in plants. In plant cells, phosphorylation of Thr11 of histone H3 is distributed along the entire length of condensed chromosome. In mammals, this modification is restricted to the centromeric region of the chromosome. Thr11 of histone H3 is phosphorylated by Dlk/ZIP kinases during mitosis.
Product Categories/Family for anti-H3T11ph antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3T11ph (Catalog #AAA388386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3T11ph antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3T11ph can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Dot Blot (DB), Western Blot (WB). Applications Validated: ChIP: 5ul per ChIP ChIP-Seq: 5ul each WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the H3T11ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3T11ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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