Chromatin Immunoprecipitation (ChIP)
(Histone H3 antibody tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Farage human lymph-node derived B cells and 4 ul Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3 binding sites.)
Rabbit anti-Human Histone H3 Polyclonal Antibody | anti-H3 antibody
Histone H3 antibody (pAb)
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3; Polyclonal Antibody; Histone H3 antibody (pAb); anti-H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3 antibody
Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB)
Application Notes
Applications Validated:
ChIP-Seq: 4ug per ChIP
WB: 0.1-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3, we also offer AbFlex Histone H3 Recombinant Antibody (rAb). For details
ChIP-Seq: 4ug per ChIP
WB: 0.1-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3, we also offer AbFlex Histone H3 Recombinant Antibody (rAb). For details
Immunogen
This antibody was raised against Recombinant Histone H3 from Xenopus laevis, which shares 99% identity to human H3 (134/136 aa are identical).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3 antibody tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Farage human lymph-node derived B cells and 4 ul Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3 binding sites.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3 antibody tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Farage human lymph-node derived B cells and 4 ul Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3 binding sites.)
Western Blot (WB)
(Histone H3 antibody tested by Western blot 20 ug HeLa cell nuclear extract was run on an SDS-PAGE gel and probed with Histone H3 antibody (pAb) at 0.5 ug/ml. MW: 17 kDa)
Western Blot (WB)
(Histone H3 antibody tested by Western blot 20 ug HeLa cell nuclear extract was run on an SDS-PAGE gel and probed with Histone H3 antibody (pAb) at 0.5 ug/ml. MW: 17 kDa)
Related Product Information for anti-H3 antibody
Short Description: Our Histone H3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq and Western blot; it has been shown to react with Human samples.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Protein Family
Similar Products
Product Notes
The H3 (Catalog #AAA389050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP-Seq: 4ug per ChIP WB: 0.1-1ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3, we also offer AbFlex Histone H3 Recombinant Antibody (rAb). For details. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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