Chromatin Immunoprecipitation (ChIP)
(Histone H3R17me2aK18ac antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed with 5 x 106 cell equivalents of chromatin from a murine HAFTL pro-B cell line and 10 ul of Histone H3R17me2aK18ac antibody. ChIP DNA was used in qPCR with a negative control primer pair (Neg) or enhancer-specific primer pairs that are “off” in B cells (Cd14) or “on” in B cells (IL7r), as indicated. This data shows that the dual modification R17me2aK18ac on Histone H3 is enriched in active enhancers. The data are presented as Fold over Input.)
Rabbit Histone H3R17me2aK18ac Polyclonal Antibody | anti-H3R17me2aK18ac antibody
Histone H3R17me2aK18ac antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Purity
Affinity Purified
Synonyms
Histone H3R17me2aK18ac; Polyclonal Antibody; Histone H3R17me2aK18ac antibody (pAb); anti-H3R17me2aK18ac antibody
Host
Rabbit
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3R17me2aK18ac antibody
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 10ul per ChIP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ChIP: 10ul per ChIP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Conjugation
Acetylated, Methylated
Immunogen
This antibody was raised against a synthetic peptide containing asymmetric dimethyl arginine 17 and acetyl lysine 18 of human Histone H3.
Abs Residue
Arg, Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3R17me2aK18ac antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed with 5 x 106 cell equivalents of chromatin from a murine HAFTL pro-B cell line and 10 ul of Histone H3R17me2aK18ac antibody. ChIP DNA was used in qPCR with a negative control primer pair (Neg) or enhancer-specific primer pairs that are “off” in B cells (Cd14) or “on” in B cells (IL7r), as indicated. This data shows that the dual modification R17me2aK18ac on Histone H3 is enriched in active enhancers. The data are presented as Fold over Input.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3R17me2aK18ac antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed with 5 x 106 cell equivalents of chromatin from a murine HAFTL pro-B cell line and 10 ul of Histone H3R17me2aK18ac antibody. ChIP DNA was used in qPCR with a negative control primer pair (Neg) or enhancer-specific primer pairs that are “off” in B cells (Cd14) or “on” in B cells (IL7r), as indicated. This data shows that the dual modification R17me2aK18ac on Histone H3 is enriched in active enhancers. The data are presented as Fold over Input.)
Western Blot (WB)
(Histone H3R17me2aK18ac antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 ug per lane) was probed with H3R17me2aK18ac (pAb) at a 1:500 dilution. Lane 1: Untreated cells. Lane 2: Cells treated with sodium butyrate.)
Western Blot (WB)
(Histone H3R17me2aK18ac antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 ug per lane) was probed with H3R17me2aK18ac (pAb) at a 1:500 dilution. Lane 1: Untreated cells. Lane 2: Cells treated with sodium butyrate.)
Dot Blot (DB)
(Histone H3R17me2aK18ac antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3R17me2aK18ac antibody for asymmetric dimethyl-arginine 17 and acetyl-lysine 18 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with H3R17me2aK18ac antibody at a 1:500 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Peptide containing asymmetric dimethyl-Arg17 and acetyl-Lys18 of Histone H3 . Lane 2: unmodified Histone H3 peptide. Lane 3: Asymmetric dimethyl-Arg17 of Histone H3 peptide. Lane 4: Acetyl-Lys18 of Histone H3 peptide.)
Dot Blot (DB)
(Histone H3R17me2aK18ac antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3R17me2aK18ac antibody for asymmetric dimethyl-arginine 17 and acetyl-lysine 18 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with H3R17me2aK18ac antibody at a 1:500 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Peptide containing asymmetric dimethyl-Arg17 and acetyl-Lys18 of Histone H3 . Lane 2: unmodified Histone H3 peptide. Lane 3: Asymmetric dimethyl-Arg17 of Histone H3 peptide. Lane 4: Acetyl-Lys18 of Histone H3 peptide.)
Related Product Information for anti-H3R17me2aK18ac antibody
Short Description: Our Histone H3R17me2aK18ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residues. Acetylation of histones occurs on lysine and is typically associated with activation of gene expression. Co-occurrence of two modifications adds an additional layer of complexity and regulation to the function of these modifications. Dual modification of H3R17me2aK18ac may play a role in enhancer function.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residues. Acetylation of histones occurs on lysine and is typically associated with activation of gene expression. Co-occurrence of two modifications adds an additional layer of complexity and regulation to the function of these modifications. Dual modification of H3R17me2aK18ac may play a role in enhancer function.
Product Categories/Family for anti-H3R17me2aK18ac antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3R17me2aK18ac (Catalog #AAA388977) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3R17me2aK18ac antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3R17me2aK18ac can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Western Blot (WB). Applications Validated: ChIP: 10ul per ChIP WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the H3R17me2aK18ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3R17me2aK18ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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