Dog Active PAI-1 functional assay ELISA Kit | SERPINE1 elisa kit
Active canine (dog) PAI-1 functional assay ELISA kit
Gene Names
SERPINE1; PAI-1
Reactivity
Dog
Synonyms
Active PAI-1 functional assay; Active canine (dog) PAI-1 functional assay ELISA kit; SERPINE1 elisa kit
Reactivity
Dog
Form/Format
Complete kit
Sequence Length
125
Source Info
Multiple
Target Info
PAI-1
Preparation and Storage
Recommended Storage: 4 degree C
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Shipping Condition: Cold pack
Minimum Shelf Life: 12 months from manufacture, see label for expiration date
Shipping Condition: Cold pack
Related Product Information for SERPINE1 elisa kit
Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of functionally active canine (dog) PAI-1 in plasma samples is easily performed with this 96 well strip format ELISA kit. The assay measures active PAI-1 in the 0.2-100 ng/ml range. Samples giving mouse PAI-1 levels above 100 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Functionally active PAI-1 reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected by the assay. After appropriate washing steps, anti-dog PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 in the samples. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Product Categories/Family for SERPINE1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14,069 Da
NCBI Official Full Name
Plasminogen activator inhibitor type 1
NCBI Official Symbol
SERPINE1
NCBI Official Synonym Symbols
PAI-1
NCBI Protein Information
plasminogen activator inhibitor 1; plasminogen activator inhibitor type 1
UniProt Protein Name
Plasminogen activator inhibitor type 1
Protein Family
UniProt Gene Name
PAI-1
UniProt Entry Name
Q7YSA1_CANFA
Uniprot Description
Protein family/group databasesMEROPS protease database<br/><a href='/database/59'>More..</a>MEROPSiI04.020.
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Product Notes
The Dog SERPINE1 pai-1 (Catalog #AAA135937) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135937 ELISA Kit recognizes Dog SERPINE1. It is sometimes possible for the material contained within the vial of "Active PAI-1 functional assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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