Chromatin Immunoprecipitation (ChIP)
(Histone H3 acetyl Lys18 antibody tested by ChIP. Top Panel: Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. Bottom Panel:Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 ul of Histone H3 acetyl Lys18 antibody. Lane 3: PCR input control.)
Rabbit Histone H3K18ac Polyclonal Antibody | anti-H3K18ac antibody
Histone H3K18ac antibody (pAb)
ChIP: 5-10ul per ChIP
ICC/IF: 1:500-1:1,000 dilution
WB: 1:2,500-1:10,000 dilution
Chromatin Immunoprecipitation (ChIP)
(Histone H3 acetyl Lys18 antibody tested by ChIP. Top Panel: Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. Bottom Panel:Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 ul of Histone H3 acetyl Lys18 antibody. Lane 3: PCR input control.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3 acetyl Lys18 antibody tested by ChIP. Top Panel: Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. Bottom Panel:Chromatin IP performed using the ChIP-IT Express Kit and 50 ul of Ready-to-ChIP HeLa Chromatin per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 ul of Histone H3 acetyl Lys18 antibody. Lane 3: PCR input control.)
Immunofluorescence (IF)
(Histone H3 acetyl Lys18 antibody tested by immunofluorescence. Top left: HeLa cells stained with Histone H3 acetyl Lys18 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Immunofluorescence (IF)
(Histone H3 acetyl Lys18 antibody tested by immunofluorescence. Top left: HeLa cells stained with Histone H3 acetyl Lys18 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.)
Western Blot (WB)
(Histone H3 acetyl Lys18 antibody tested by Western blot. A549 whole-cell extract (20 ug per lane) probed with Histone H3 acetyl Lys18 antibody (1:5,000 dilution). Lane 1: Untreated cells. Lane 2: Cells treated with Trichostatin A.)
Western Blot (WB)
(Histone H3 acetyl Lys18 antibody tested by Western blot. A549 whole-cell extract (20 ug per lane) probed with Histone H3 acetyl Lys18 antibody (1:5,000 dilution). Lane 1: Untreated cells. Lane 2: Cells treated with Trichostatin A.)
Dot Blot (DB)
(Histone H3 acetyl Lys18 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 antibody for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl-Lys14 peptide. Lane 7: acetyl-Lys18 peptide (this is a different peptide sequence as the one in lane 3 which also contains acetyl-Lys18). Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide.)
Dot Blot (DB)
(Histone H3 acetyl Lys18 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 antibody for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl-Lys14 peptide. Lane 7: acetyl-Lys18 peptide (this is a different peptide sequence as the one in lane 3 which also contains acetyl-Lys18). Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide.)
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
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Product Notes
The H3K18ac (Catalog #AAA388502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K18ac antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K18ac can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Applications Validated: ChIP: 5-10ul per ChIP ICC/IF: 1:500-1:1,000 dilution WB: 1:2,500-1:10,000 dilution. Researchers should empirically determine the suitability of the H3K18ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K18ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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