Rabbit Histone H3R2me2s Polyclonal Antibody | anti-H3R2me2s antibody

Histone H3R2me2s (symmetric) antibody (pAb)

Cat. Num. AAA388548

• Reactivity: Human, Wide Range Predicted
• Applications: Dot Blot, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: Histone H3R2me2s; Polyclonal Antibody; Histone H3R2me2s (symmetric) antibody (pAb); anti-H3R2me2s antibody

• Host: Rabbit
• Reactivity: Human, Wide Range Predicted
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-H3R2me2s antibody: Dot Blot (DB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
• Conjugation: Methylated
• Immunogen: This Histone H3 dimethyl Arg2 (H3R2me2s) antibody was raised against a peptide containing dimethyl-Arg2 (symmetric) of human histone H3.
• Abs Residue: Arg
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(Histone H3R2me2s (symmetric) antibody (pAb) tested by Western blot. Western blot performed using Histone H3R2me2s (symmetric) antibody (pAb) at a dilution of 1:500. Lane 1: Nuclear extract from HEK293 cells (25 ug). Lane 2: Nuclear extract from HeLa cells (20 ug).)

Dot Blot (DB)

(Histone H3R2me2s (symmetric) antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3R2me2s (symmetric) antibody (pAb) for dimethyl-arginine 2 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg2 symmetric pAb at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmodified Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (symmetric). Lane 4: dimethyl-Arg2 H3 (asymmetric). Lane 5: unmodified Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (symmetric). Lane 8: dimethyl-Arg8 H3 (asymmetric).Bottom Panel: Lane 1: unmodified Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (symmetric). Lane 4: dimethyl-Arg17 H3 (asymmetric). Lane 5: unmodified Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (symmetric). Lane 8: dimethyl-Arg26 H3 (asymmetric).)

Related Product Information for anti-H3R2me2s antibody

Short Description: Our Histone H3R2me2s (symmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence, Immunohistochemistry and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.

Product Categories/Family for anti-H3R2me2s antibody

Primary Antibodies

NCBI and Uniprot Product Information

• Molecular Weight: 17kDa