Western Blot (WB)
(Western blot analysis of extracts from HeLa, H-4-II-E and COS cells using MacroH2A1.2 antibody.)
Rabbit Histone H2A1.2, Macro Polyclonal Antibody | anti-H2A1-2 antibody
Histone H2A1.2, Macro
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Histone H2A1.2; Macro; Polyclonal Antibody; Anti -Histone H2A1.2; anti-H2A1-2 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MacroH2A1.2. Species crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Applicable Applications for anti-H2A1-2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot and Immunofluorescence (IF-IC).
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunofluorescence (IF-IC): 1:200.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunofluorescence (IF-IC): 1:200.
Immunogen
Synthetic peptide corresponding to the human MacroH2A1.2 protein (MacroH2A1, isoform 2) (KLH- coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from HeLa, H-4-II-E and COS cells using MacroH2A1.2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa, H-4-II-E and COS cells using MacroH2A1.2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, either untransfected or transfected with expression constructs for MacroH2A1.1 or MacroH2A1.2, using MacroH2A1.2 antibody (upper) and MacroH2A1.1 antibody (lower).)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, either untransfected or transfected with expression constructs for MacroH2A1.1 or MacroH2A1.2, using MacroH2A1.2 antibody (upper) and MacroH2A1.1 antibody (lower).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using MacroH2A1.2 antibody (green). Actin filaments were labeled using DY-554 phalloidin (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HeLa cells using MacroH2A1.2 antibody (green). Actin filaments were labeled using DY-554 phalloidin (red).)
Related Product Information for anti-H2A1-2 antibody
Histone macroH2A1 and macroH2A2 comprise a family of variant histone H2A proteins. MacroH2A1 exists as two distinct isoforms due to alternative splicing of a single gene; macroH2A1.1 levels accumulate throughout differentiation and development while macroH2A1.2 shows a constant level of expression (1). MacroH2A1 and macroH2A2 are encoded by completely distinct genes located on separate chromosomes (2,3). Both macroH2A1 and macroH2A2 proteins contain an amino-terminal histone-like region with 64% sequence identity to canonical histone H2A, in addition to a carboxy-terminal "macro" domain (1-3). MacroH2A1 and macroH2A2 are enriched in facultative heterochromatin, including inactivated X chromosomes in mammalian females and senescence-associated heterochro- matin foci (2-5). Both act to repress gene transcription by inhibiting the binding of transcription factors to chromatin, the acetylation of histones by p300, and the chromatin-remodeling activities of SWI/SNF and ACF (6,7). The macro domain of macroH2A1.1 binds to ADP-ribose and functions to recruit macroH2A1.1 to activated PARP at sites of DNA damage, where it mediates chromatin rearrangements to lo- cally regulate the DNA damage response (8). MacroH2A1.2 and macroH2A2 do not bind poly-ADP-ribose and are not recruited to sites of activated PARP (8).
Product Categories/Family for anti-H2A1-2 antibody
NCBI and Uniprot Product Information
Uniprot Description
Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA
By similarity. RuleBase RU003556
Subcellular location: Nucleus
By similarity RuleBase RU000527.
Sequence similarities: Belongs to the histone H2A family. RuleBase RU003767
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Product Notes
The Histone H2A1.2, Macro h2a1-2 (Catalog #AAA620652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A1.2, Macro reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A1.2, Macro can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Western Blot and Immunofluorescence (IF-IC). Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:200. Researchers should empirically determine the suitability of the Histone H2A1.2, Macro h2a1-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A1.2, Macro, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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