Rabbit anti-Human BRDT Polyclonal Antibody | anti-BRDT antibody

BRDT, NT (BRDT, Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein) (FITC)

Cat. Num. AAA6278871

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BRDT; Polyclonal Antibody; NT (BRDT; Bromodomain testis-specific protein; Cancer/testis antigen 9; RING3-like protein) (FITC); RING3-like protein; anti-BRDT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BRDT antibody: FLISA, Western Blot (WB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BRDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~38 amino acids from the N-terminal region of human BRDT.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRDT N-term western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BRDT antibody detected the BRDT protein (arrow).)

Product Categories/Family for anti-BRDT antibody

Antibodies; Cancer Markers

References

Pivot-Pajot, C., et al., Mol. Cell. Biol. 23(15):5354-5365 (2003). Dhalluin, C., et al., Nature 399(6735):491-496 (1999). Jones, M.H., et al., Genomics 45(3):529-534 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 74705454
• UniProt Accession #: O14789; Q05DQ4; Q6P5T1; Q7Z4A6; Q8IWI6; A6NF68; B7Z811; B7Z890; B7ZAX7; D3DT32
• Molecular Weight: 109,506 Da
• UniProt Protein Name: Bromodomain testis-specific protein
• Protein Family: Bromodomain testis-specific protein
• UniProt Gene Name: BRDT
• UniProt Synonym Gene Names: CT9

Uniprot Description

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.