Mouse Jumonji Domain Containing Protein 1B Monoclonal Antibody | anti-KDM3B antibody

Jumonji Domain Containing Protein 1B (JMJD1B, JmjC Domain-containing Histone Demethylation Protein 2B, JHDM2B, KDM3B, 5qNCA, Nuclear Protein 5qNCA)

Cat. Num. AAA607155

• Gene Names: KDM3B; 5qNCA; NET22; C5orf7; JMJD1B; KIAA1082
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Jumonji Domain Containing Protein 1B; Monoclonal Antibody; Jumonji Domain Containing Protein 1B (JMJD1B; JmjC Domain-containing Histone Demethylation Protein 2B; JHDM2B; KDM3B; 5qNCA; Nuclear Protein 5qNCA); Anti -Jumonji Domain Containing Protein 1B (JMJD1B; anti-KDM3B antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10E35
• Specificity: Recognizes human JMJD1B (6A1-1F5). Species crossreactivity: mouse, rat, monkey.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and 0.02% sodium azide.

• Applicable Applications for anti-KDM3B antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence (IF-IC).; Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X; TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50; Immunofluorescence (IF-IC): 1:200
• Immunogen: Recombinant protein derived from the sequence of human JMJD1B.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from NCCIT cells using MBS607155.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS607155 (green). Actin filaments were labeled using DY-554 phalloidin (red).)

Related Product Information for anti-KDM3B antibody

The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and trimethyl lysine residues via an oxidative reaction that requires iron and -ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JH- DM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).

Product Categories/Family for anti-KDM3B antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 119582546
• NCBI GeneID: 51780
• UniProt Accession #: Q7LBC6; Q9BVH6; Q9BW93; Q9BZ52; Q9NYF4; Q9UPS0; A6H8X7
• Molecular Weight: 191,581 Da
• NCBI Official Full Name: jumonji domain containing 1B, isoform CRA_b
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 3B
• NCBI Official Symbol: KDM3B
• NCBI Official Synonym Symbols: 5qNCA; NET22; C5orf7; JMJD1B; KIAA1082
• NCBI Protein Information: lysine-specific demethylase 3B; OTTHUMP00000224009; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
• UniProt Protein Name: Lysine-specific demethylase 3B
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM3B
• UniProt Synonym Gene Names: C5orf7; JHDM2B; JMJD1B; KIAA1082
• UniProt Entry Name: KDM3B_HUMAN

Uniprot Description

JMJD1B: a ubiquitous protein demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Three alternatively spliced isoforms have been reported.

Protein type: Demethylase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm

Molecular Function: dioxygenase activity; metal ion binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification