Rabbit HBP1 Polyclonal Antibody | anti-HBP1 antibody

HBP1 (L468) Polyclonal Antibody

Cat. Num. AAA3001815

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HBP1; Polyclonal Antibody; HBP1 (L468) Polyclonal Antibody; HMG box-containing protein 1; HMG box transcription factor 1; High mobility group box transcription factor 1; anti-HBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HBP1 (L468) polyclonal antibody detects endogenous levels of HBP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 524

• Applicable Applications for anti-HBP1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 441-490 of Human HBP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of HBP1 (L468) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-HBP1 antibody

The HMG-box protein-1 (HBP1) is a member of the HMG family of transcription factors, which are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, that mediates DNA binding. HBP1 binds to the tumor suppressor proteins Rb and p130 and initiates cell cycle arrest. Terminal cell differentiation requires this initial cell cycle arrest followed by the coordinated expression of genes defined as tissue-specifc markers. Along with initiating the commitment to cell differentiation, the continued activity of HBP1 abrogates the expression of tissue-specific genes by associating with the MyoD proteins. In muscle cell differentiation, the MyoD family of transcription factors, which include Myf5, MyoD and myogenein, induce the expression of these cell-type specific proteins and contribute to the development of cell phenotypes. The progression of terminal differentiation is, therefore, dependent on both a decrease in HBP1 activity and the corresponding activation of MyoD-induced gene transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 346716352
• NCBI GeneID: 26959
• NCBI Accession #: NP_001231191.1
• NCBI GenBank Nucleotide #: NP_001231191.1
• UniProt Accession #: O60381
• Molecular Weight: ~ 58, 80kDa
• NCBI Official Full Name: HMG box-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: HMG-box transcription factor 1
• NCBI Official Symbol: HBP1
• NCBI Protein Information: HMG box-containing protein 1
• UniProt Protein Name: HMG box-containing protein 1
• Protein Family: HMG box-containing protein
• UniProt Gene Name: HBP1
• UniProt Entry Name: HBP1_HUMAN

Uniprot Description

HBP1: Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding; RNA binding

Biological Process: Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of potassium ion transport; cell cycle arrest