HBP1 recombinant protein

HBP1 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121626

• Purity: >95% as determined by SDS-PAGE
• Synonyms: HBP1; HBP1 Protein; Human; Recombinant (GST Tag); Human HBP1 Protein (GST Tag); HMG-box transcription factor 1; HBP1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro208-Phe345

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mature form of human HBP1 (O60381-1)(Pro208-Phe345) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HBP1 recombinant protein

Background: HBP1 is a sequence-specific DNA-binding transcription factor. It is involved in many biological processes. It was reported that HBP1 binds to p16(INK4A) promoter and activates p16(INK4A) expression. We found that trichostatin A (TSA), an inhibitor of HDAC (histone deacetylase), induces p16(INK4A) expression in an HBP1-dependent manner. HBP1 activates or represses the expression of some specific genes during cell growth and differentiation. HBP1 was acetylated by p3/CBP in two regions: repression domain (K297/35/37) and P domain (K171/419). HBP1 acetylation after TSA treatment was confirmed by immunoprecipitation assay. HBP1 interacted with histone acetyltransferase p3 and CREB-binding protein (CBP) and also recruited p3/CBP to p16(INK4A) promoter. HBP1 acetylation at K419 plays an important role in HBP1-induced p16(INK4A) expression.

References

Tevosian SG. et al., 1997, Genes Dev. 11 (3): 383-96. Lavender P. et al., 1997, Oncogene. 14 (22): 2721-8. Swanson. et al., 2004, Nat Struct Mol Biol. 11 (8): 738-46.

NCBI and Uniprot Product Information

• NCBI GI #: 346716352
• NCBI GeneID: 26959
• NCBI Accession #: NP_001231191.1
• NCBI GenBank Nucleotide #: NM_001244262.1
• UniProt Accession #: O60381; Q8TBM1; Q8TE93; Q96AJ2; B3KVB7
• Molecular Weight: 57,645 Da
• NCBI Official Full Name: HMG box-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: HMG-box transcription factor 1
• NCBI Official Symbol: HBP1
• NCBI Protein Information: HMG box-containing protein 1; HMG box transcription factor 1; high mobility group box transcription factor 1
• UniProt Protein Name: HMG box-containing protein 1
• Protein Family: HMG box-containing protein
• UniProt Gene Name: HBP1
• UniProt Entry Name: HBP1_HUMAN

Uniprot Description

HBP1: Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding; RNA binding

Biological Process: Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of potassium ion transport; cell cycle arrest