Western Blot (WB)
(Western Blot analysis of extracts from Hela cells using p21Cip1 antibody and the same antibody preincubated with blocking peptide.)
Rabbit p21Cip1 Polyclonal Antibody | anti-p21Cip1 antibody
p21Cip1 antibody
Applications
Western Blot
Purity
p21Cip1 antibody was purified by immunoaffinity chromatography.
Synonyms
p21Cip1; Polyclonal Antibody; p21Cip1 antibody; Polyclonal p21Cip1; Anti-p21Cip1; pCip1 21; CDK-interacting protein 1; CDKN1A; pCip1-21; Ab 145; CDKN1; CDK-interaction protein 1; CAP20; anti-p21Cip1 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
p21Cip1 antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-p21Cip1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Biological Significance
p21Cip1 a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.
Cross-Reactivity
Human
Immunogen
p21Cip1 antibody was raised in Rabbit using the peptide sequence around aa 143~147 (R-Q-T-S-M) derived from Human p21Cip1 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts from Hela cells using p21Cip1 antibody and the same antibody preincubated with blocking peptide.)
Western Blot (WB)
(Western Blot analysis of extracts from Hela cells using p21Cip1 antibody and the same antibody preincubated with blocking peptide.)
Related Product Information for anti-p21Cip1 antibody
Rabbit polyclonal p21Cip1 antibody
Product Categories/Family for anti-p21Cip1 antibody
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Product Notes
The p21Cip1 (Catalog #AAA837463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's p21Cip1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the p21Cip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21Cip1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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