Rabbit GRM5 / MGLUR5 Polyclonal Antibody | anti-GRM5 antibody

Rabbit Polyclonal to Human GRM5 / MGLUR5

Cat. Num. AAA243804

• Gene Names: GRM5; mGlu5; GPRC1E; MGLUR5; PPP1R86
• Reactivity: Bat, Gibbon, Bovine, Dog, Horse, Human, Monkey, Mouse, Rat; Predicted Reactivity: Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: GRM5 / MGLUR5; Polyclonal Antibody; Rabbit Polyclonal to Human GRM5 / MGLUR5; Anti-GRM5 / MGLUR5 Antibody (C-Terminus) IHC-plus; GRM5; GPRC1E; MGLUR5; Mglur5b; MGlu5; Human GRM5; anti-GRM5 antibody

• Host: Rabbit
• Reactivity: Bat, Gibbon, Bovine, Dog, Horse, Human, Monkey, Mouse, Rat; Predicted Reactivity: Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GRM5 / MGLUR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PLEC (45%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1180

• Applicable Applications for anti-GRM5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (4 - 13 ug/ml), WB
• Target Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from C-terminus of human GRM5 / MGLUR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%); Platypus (95%).
• Immunogen Type: Synthetic peptide
• Immunogen: GRM5 / MGLUR5 antibody was raised against synthetic 20 amino acid peptide from C-terminus of human GRM5 / MGLUR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%); Platypus (95%); Opossum, Turkey, Chicken, Lizard (90%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-GRM5 / MGLUR5 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-GRM5 / MGLUR5 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-GRM5 antibody

Group I of the Metabotropic Glutamate Receptors includes GRM1 and GRM5, receptors that have been shown to activate phospholipase C. Alternative splice variants of GRM5 have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain.

Product Categories/Family for anti-GRM5 antibody

Family: GPCR; Subfamily: Metabotropic glutamate

NCBI and Uniprot Product Information

• NCBI GI #: 4504143
• NCBI GeneID: 2915
• NCBI Accession #: NP_000833.1
• NCBI GenBank Nucleotide #: NM_000842.4
• UniProt Accession #: P41594; Q6J164
• Molecular Weight: 105,432 Da
• NCBI Official Full Name: metabotropic glutamate receptor 5 isoform b
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 5
• NCBI Official Symbol: GRM5
• NCBI Official Synonym Symbols: mGlu5; GPRC1E; MGLUR5; PPP1R86
• NCBI Protein Information: metabotropic glutamate receptor 5; protein phosphatase 1, regulatory subunit 86
• UniProt Protein Name: Metabotropic glutamate receptor 5
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM5
• UniProt Synonym Gene Names: GPRC1E; MGLUR5; mGluR5
• UniProt Entry Name: GRM5_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

mGluR5: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system and generates a calcium-activated chloride current. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q14.3

Cellular Component: integral to plasma membrane; cytoplasm; postsynaptic density; dendritic spine; plasma membrane; dendritic shaft

Molecular Function: G-protein coupled receptor activity; glutamate receptor activity; A2A adenosine receptor binding

Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; regulation of synaptic transmission, glutamatergic; locomotory behavior; learning; positive regulation of long-term neuronal synaptic plasticity; protein amino acid phosphorylation; synaptic transmission; negative regulation of locomotion; regulation of transcription, DNA-dependent; metabotropic glutamate receptor, phospholipase C activating pathway; protein kinase C activation; metabotropic glutamate receptor signaling pathway; activation of MAPKKK activity; cognition