Rabbit mGluR5/GRM5 Polyclonal Antibody | anti-GRM5 antibody

Anti-mGluR5/GRM5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101762

• Gene Names: GRM5; mGlu5; GPRC1E; MGLUR5; PPP1R86
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: mGluR5/GRM5; Polyclonal Antibody; Anti-mGluR5/GRM5 Antibody; Rabbit Polyclonal; GRM5 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-GPRC1E Antibody; Anti-mGlu5 Antibody; Anti-MGLUR5 Antibody; Anti-PPP1R86 Antibody; anti-GRM5 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human mGluR5/GRM5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GRM5 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human GRM5
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GRM5, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human GRM5 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-GRM5 antibody

Metabotropic glutamate receptor 5 (mGluR5/GRM5) is a Galphaq/11-coupled receptor, mainly found at the postsynaptic site. mGluR5 stimulation leads to the activation of phospholipase Cbeta1 (PLCbeta), promoting diacylglicerol (DAG) and inositol 1, 4, 5-trisphosphate (IP3) formation, which leads to the release of Ca2+ from the intracellular stores and the activation of protein kinases, including protein kinase C. Additionally, stimulation of mGluR5 also triggers the activation of other cell signaling pathways that are important for cell proliferation and survival, such as the activation of the extracellular signal regulated protein kinase (ERK) and AKT. mGluR5 has also been implicated in the pathology of other neurodegenerative diseases, including Alzheimer and Parkinson's disease, as well as in physiological processes, including memory and motor coordination.

References

Kelly E, et al. (2018) Mglur5 modulation of behavioral and epileptic phenotypes in a mouse model of tuberous sclerosis complex. Neuropsychopharmacology 43 (6): 1457-1465.Carvalho TG, et al. (2017) Mglur5, cb1 and neuroprotection. Oncotarget 8 (3): 3768-3769.Aloisi E, et al. (2017) Altered surface mglur5 dynamics provoke synaptic nmdar dysfunction and cognitive defects in fmr1 knockout mice. Nat Commun 8 (1): 1103.

NCBI and Uniprot Product Information

• NCBI GI #: 4504143
• NCBI GeneID: 2915
• NCBI Accession #: NP_000833.1
• NCBI GenBank Nucleotide #: NM_000842.4
• UniProt Accession #: P41594; Q6J164
• Molecular Weight: 132469 MW
• NCBI Official Full Name: metabotropic glutamate receptor 5 isoform b
• NCBI Official Synonym Full Names: glutamate metabotropic receptor 5
• NCBI Official Symbol: GRM5
• NCBI Official Synonym Symbols: mGlu5; GPRC1E; MGLUR5; PPP1R86
• NCBI Protein Information: metabotropic glutamate receptor 5
• UniProt Protein Name: Metabotropic glutamate receptor 5
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM5
• UniProt Synonym Gene Names: GPRC1E; MGLUR5; mGluR5

NCBI Description

This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.