Rabbit GPR3 Polyclonal Antibody | anti-GPR3 antibody

Rabbit Polyclonal to Human GPR3

Cat. Num. AAA240901

• Gene Names: GPR3; ACCA
• Reactivity: Gibbon, Bovine, Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Rat, Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GPR3; Polyclonal Antibody; Rabbit Polyclonal to Human GPR3; Anti-GPR3 Antibody (Extracellular Domain) IHC-plus; ACCA; ACCA orphan receptor; G protein-coupled receptor 3; GPCR3; Hacca; G-protein coupled receptor 3; Human GPR3; anti-GPR3 antibody

• Host: Rabbit
• Reactivity: Gibbon, Bovine, Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Rat, Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TKT (44%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 330

• Applicable Applications for anti-GPR3 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (40 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Elephant, Panda, Rabbit (100%); Rat, Pig (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPR3 antibody was raised against synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Elephant, Panda, Rabbit (100%); Rat, Pig (94%); Mouse, Hamster, Horse (88%).
• Antigen Modification: Extracellular Domain
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-GPR3 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-GPR3 antibody

GPR3 has been identified as a constitutively active lysophospholipid/lysosphingolipid receptor. GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle where GPR3 maintains meiotic arrest in mouse oocytes. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype Mice deficient in GPR3 exhibit premature ovarian failure due to early oocyte aging (Ledent et al. 2005).

Product Categories/Family for anti-GPR3 antibody

Family: GPCR; Subfamily: Lysophospholipid/Lysosphingolipid

NCBI and Uniprot Product Information

• NCBI GI #: 4885325
• NCBI GeneID: 2827
• NCBI Accession #: NP_005272.1
• NCBI GenBank Nucleotide #: NM_005281.3
• UniProt Accession #: P46089; A8K570
• Molecular Weight: 35,010 Da
• NCBI Official Full Name: G-protein coupled receptor 3
• NCBI Official Synonym Full Names: G protein-coupled receptor 3
• NCBI Official Symbol: GPR3
• NCBI Official Synonym Symbols: ACCA
• NCBI Protein Information: G-protein coupled receptor 3; ACCA orphan receptor; adenylate cyclase constitutive activator
• UniProt Protein Name: G-protein coupled receptor 3
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: GPR3
• UniProt Synonym Gene Names: ACCA
• UniProt Entry Name: GPR3_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR3: Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons and neurite outgrowth. Maintains also meiotic arrest in oocytes. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; regulation of meiosis; positive regulation of cAMP metabolic process; G-protein signaling, adenylate cyclase activating pathway