Mouse anti-Human GPR3 Monoclonal Antibody | anti-GPR3 antibody

Mouse Monoclonal [clone 3B4-G3] (IgG2a,k) to Human GPR3

Cat. Num. AAA245574

• Gene Names: GPR3; ACCA
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: GPR3; Monoclonal Antibody; Mouse Monoclonal [clone 3B4-G3] (IgG2a; k) to Human GPR3; Anti-GPR3 Antibody (clone 3B4-G3) IHC-plus; ACCA; ACCA orphan receptor; G protein-coupled receptor 3; GPCR3; Hacca; G-protein coupled receptor 3; Human GPR3; anti-GPR3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3B4-G3
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Sequence Length: 330

• Applicable Applications for anti-GPR3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), RNA interference
• Application Notes: ELISA, IHC-P (5 ug/ml), RNAi, WB; Usage: Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. RNAi Knockdown (Antibody validated).
• Target Species: Human
• Immunogen Description: GPR3 (AAH32702, 1 a.a. ~ 331 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: GPR3 antibody was raised against gPR3 (AAH32702, 1 a.a. ~ 331 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody clone 3B4-G3.)

Western Blot (WB)

(GPR3 monoclonal antibody, clone 3B4-G3 Western blot of GPR3 expression in Jurkat.)

RNA interference

(Western blot of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody, clone 3B4-G3. GAPDH (36.1 kD) used as specificity a.)

ELISA (EIA)

(Detection limit for recombinant GST tagged GPR3 is approximately 0.03 ng/ml as a capture antibody.)

Related Product Information for anti-GPR3 antibody

GPR3 has been identified as a constitutively active lysophospholipid/lysosphingolipid receptor. GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle where GPR3 maintains meiotic arrest in mouse oocytes. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype Mice deficient in GPR3 exhibit premature ovarian failure due to early oocyte aging (Ledent et al. 2005).

Product Categories/Family for anti-GPR3 antibody

Family: GPCR; Subfamily: Lysophospholipid/Lysosphingolipid

NCBI and Uniprot Product Information

• NCBI GI #: 4885325
• NCBI GeneID: 2827
• NCBI Accession #: NP_005272.1
• NCBI GenBank Nucleotide #: NM_005281.3
• UniProt Accession #: P46089; A8K570
• Molecular Weight: 35,010 Da
• NCBI Official Full Name: G-protein coupled receptor 3
• NCBI Official Synonym Full Names: G protein-coupled receptor 3
• NCBI Official Symbol: GPR3
• NCBI Official Synonym Symbols: ACCA
• NCBI Protein Information: G-protein coupled receptor 3; ACCA orphan receptor; adenylate cyclase constitutive activator
• UniProt Protein Name: G-protein coupled receptor 3
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: GPR3
• UniProt Synonym Gene Names: ACCA
• UniProt Entry Name: GPR3_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR3: Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons and neurite outgrowth. Maintains also meiotic arrest in oocytes. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell cycle regulation; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; regulation of meiosis; positive regulation of cAMP metabolic process; G-protein signaling, adenylate cyclase activating pathway