Rabbit anti-Human GPR3 Polyclonal Antibody | anti-GPR3 antibody

GPR3, CT (G-protein Coupled Receptor 3, ACCA Orphan Receptor, ACCA)

Cat. Num. AAA642070

• Gene Names: GPR3; ACCA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GPR3; Polyclonal Antibody; CT (G-protein Coupled Receptor 3; ACCA Orphan Receptor; ACCA); Anti -GPR3; anti-GPR3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human GPR3.Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-GPR3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 13aa from human GPR3 at C-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2ug/ml.)

Immunohistochemistry (IHC)

(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-GPR3 antibody

GPR3 has been identified as a constitutively active lysophospholipid/lysosphingolipid receptor. GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle where GPR3 maintains meiotic arrest in mouse oocytes. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype Mice deficient in GPR3 exhibit premature ovarian failure due to early oocyte aging (Ledent et al. 2005).

Product Categories/Family for anti-GPR3 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4885325
• NCBI GeneID: 2827
• NCBI Accession #: NP_005272.1
• NCBI GenBank Nucleotide #: NM_005281.3
• UniProt Accession #: P46089; A8K570
• Molecular Weight: 35,010 Da
• NCBI Official Full Name: G-protein coupled receptor 3
• NCBI Official Synonym Full Names: G protein-coupled receptor 3
• NCBI Official Symbol: GPR3
• NCBI Official Synonym Symbols: ACCA
• NCBI Protein Information: G-protein coupled receptor 3; ACCA orphan receptor; adenylate cyclase constitutive activator
• UniProt Protein Name: G-protein coupled receptor 3
• Protein Family: G-protein coupled receptor
• UniProt Gene Name: GPR3
• UniProt Synonym Gene Names: ACCA
• UniProt Entry Name: GPR3_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR3: Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons and neurite outgrowth. Maintains also meiotic arrest in oocytes. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Cell cycle regulation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; regulation of meiosis; positive regulation of cAMP metabolic process; G-protein signaling, adenylate cyclase activating pathway