Typical Testing Data/Standard Curve (for reference only)
Rabbit Alpha-amylase 2B (AMY2B) ELISA Kit | AMY2B elisa kit
Rabbit Alpha-amylase 2B (AMY2B) ELISA Kit
Gene Names
AMY2B; HXA; AMY2; AMY3
Reactivity
Rabbit
Synonyms
Alpha-amylase 2B (AMY2B); Rabbit Alpha-amylase 2B (AMY2B) ELISA Kit; AMY2B elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMY2B elisa kit
Intended Uses: This AMY2B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit AMY2B.
Principle of the Assay: AMY2B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMY2B antibody and an AMY2B-HRP conjugate. The assay sample and buffer are incubated together with AMY2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY2B concentration since AMY2B from samples and AMY2B-HRP conjugate compete for the anti-AMY2B antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY2B from the sample, fewer sites are left to bind AMY2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY2B concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AMY2B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMY2B antibody and an AMY2B-HRP conjugate. The assay sample and buffer are incubated together with AMY2B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY2B concentration since AMY2B from samples and AMY2B-HRP conjugate compete for the anti-AMY2B antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY2B from the sample, fewer sites are left to bind AMY2B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY2B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AMY2B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,710 Da
NCBI Official Full Name
alpha-amylase 2B
NCBI Official Synonym Full Names
amylase, alpha 2B (pancreatic)
NCBI Official Symbol
AMY2B
NCBI Official Synonym Symbols
HXA; AMY2; AMY3
NCBI Protein Information
alpha-amylase 2B; glycogenase; carcinoid alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase 2B
UniProt Protein Name
Alpha-amylase 2B
Protein Family
UniProt Gene Name
AMY2B
UniProt Entry Name
AMY2B_HUMAN
NCBI Description
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jun 2013]
Uniprot Description
AMY2B: Monomer. Belongs to the glycosyl hydrolase 13 family.
Protein type: EC 3.2.1.1; Secreted; Carbohydrate Metabolism - starch and sucrose; Hydrolase; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p21
Molecular Function: alpha-amylase activity; metal ion binding
Biological Process: digestion; carbohydrate metabolic process
Research Articles on AMY2B
Similar Products
Product Notes
The Rabbit AMY2B amy2b (Catalog #AAA7225061) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7225061 ELISA Kit recognizes Rabbit AMY2B. It is sometimes possible for the material contained within the vial of "Alpha-amylase 2B (AMY2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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