Western Blot (WB)
(Staining (0.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat UBE2L3 Polyclonal Antibody | anti-UBE2L3 antibody
Goat anti-UBE2L3 Antibody
Western Blot: Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Staining (0.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
UBE2L3: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1. Ubiquitous, with highest expression in testis. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ligase; Nuclear receptor co-regulator; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex
Molecular Function: ATP binding; enzyme binding; protein binding; transcription coactivator activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: cell proliferation; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein modification process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process
Research Articles on UBE2L3
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Product Notes
The UBE2L3 ube2l3 (Catalog #AAA420751) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-UBE2L3 Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2L3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the UBE2L3 ube2l3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EFTKKYGEKR PVD. It is sometimes possible for the material contained within the vial of "UBE2L3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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