Western Blot (WB)
(Western blot analysis of extracts from Human pancreas, using GPAT-2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit GPAT-2 Polyclonal Antibody | anti-GPAT2 antibody
GPAT-2 Antibody
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from Human pancreas, using GPAT-2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human pancreas, using GPAT-2 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612342 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612342) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612342 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612342) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Subcellular Location: Mitochondrion outer membrane>Multi-pass membrane protein.
Subunit Structure: Interacts with PIWIL2.
Similarity: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.Belongs to the GPAT/DAPAT family.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)88kDa.
Uniprot Description
GPAT2: Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Belongs to the GPAT/DAPAT family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; Membrane protein, integral; EC 2.3.1.15; Membrane protein, multi-pass; Mitochondrial; Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 2q11.1
Cellular Component: integral to membrane; mitochondrial outer membrane; mitochondrion
Molecular Function: glycerol-3-phosphate O-acyltransferase activity
Biological Process: CDP-diacylglycerol biosynthetic process; glycerol-3-phosphate metabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process
Research Articles on GPAT2
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Product Notes
The GPAT2 gpat2 (Catalog #AAA9612342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPAT-2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's GPAT-2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the GPAT2 gpat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPAT-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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