Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

CD4 Monoclonal Antibody

Cat. Num. AAA2548878

• Gene Names: CD4; CD4mut
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Protein A Purification
• Synonyms: CD4; Monoclonal Antibody; CD4 Monoclonal Antibody; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; anti-CD4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G1
• Purity/Purification: Protein A Purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-CD4 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: IHC: 1:100-1:300; IF: 1:50-200
• Immunogen: Synthetic Peptide
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human stomach tissue using CD4 Monoclonal Antibody at dilution of 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Mouse brain tissue using CD4 Monoclonal Antibody at dilution of 1:200.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Mouse colonic tissue with CD4 Monoclonal Antibody.)

Related Product Information for anti-CD4 antibody

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: clathrin-coated vesicle membrane; early endosome; endoplasmic reticulum lumen; endoplasmic reticulum membrane; external side of plasma membrane; integral component of plasma membrane; membrane raft; plasma membrane; T cell receptor complex

Molecular Function: coreceptor activity; enzyme binding; extracellular matrix structural constituent; identical protein binding; immunoglobulin binding; interleukin-16 binding; interleukin-16 receptor activity; MHC class II protein binding; protein binding; protein homodimerization activity; protein kinase binding; receptor activity; transmembrane receptor activity; viral receptor activity; zinc ion binding

Biological Process: adaptive immune response; cell adhesion; cell surface receptor signaling pathway; cytokine production; defense response to Gram-negative bacterium; entry into host cell; enzyme linked receptor protein signaling pathway; fusion of virus membrane with host plasma membrane; immune response; induction by virus of host cell-cell fusion; maintenance of cellular protein localization; positive regulation of calcium ion transport into cytosol; positive regulation of calcium-mediated signaling; positive regulation of ERK1 and ERK2 cascade; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-2 biosynthetic process; positive regulation of kinase activity; positive regulation of MAPK cascade; positive regulation of monocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; positive regulation of protein phosphorylation; positive regulation of T cell proliferation; positive regulation of transcription, DNA-templated; positive regulation of virion penetration into host cell; regulation of calcium ion transport; regulation of defense response to virus by virus; regulation of T cell activation; response to estradiol; response to vitamin D; signal transduction; T cell costimulation; T cell differentiation; T cell receptor signaling pathway; T cell selection; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Okt4 Epitope Deficiency